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Dose Uptake of Platinum- and Ruthenium-based Compound Exposure in Zebrafish by Inductively Coupled Plasma Mass Spectrometry with Broader Applications. | LitMetric

AI Article Synopsis

  • - Metals and metal-based compounds are significant due to their diverse effects, ranging from being toxic to serving as treatments in chemotherapy, making the study of their toxicokinetics crucial.
  • - Zebrafish are increasingly used as a model for studying these compounds because they allow for higher-throughput research compared to rodents; however, waterborne dosing methods present challenges in accurately determining internal dosages of these metals.
  • - A new technique was developed to analyze zebrafish larval tissue for metal concentrations using inductively coupled plasma mass spectrometry (ICPMS), which provides a better understanding of how much of these compounds are absorbed and their potential biological impact.

Article Abstract

Metals and metal-based compounds comprise multifarious pharmaco-active and toxicological xenobiotics. From heavy metal toxicity to chemotherapeutics, the toxicokinetics of these compounds have both historical and modern-day relevance. Zebrafish have become an attractive model organism in elucidating pharmaco- and toxicokinetics in environmental exposure and clinical translation studies. Although zebrafish studies have the benefit of being higher-throughput than rodent models, there are several significant constraints to the model. One such limitation is inherent in the waterborne dosing regimen. Water concentrations from these studies cannot be extrapolated to provide reliable internal dosages. Direct measurements of the metal-based compounds allow for a better correlation with compound-related molecular and biological responses. To overcome this limitation for metals and metal-based compounds, a technique was developed to digest zebrafish larval tissue after exposure and quantify metal concentrations within tissue samples by inductively coupled plasma mass spectrometry (ICPMS). ICPMS methods were used to determine the metal concentrations of platinum (Pt) from cisplatin and ruthenium (Ru) from several novel Ru-based chemotherapeutics in zebrafish tissue. Additionally, this protocol distinguished concentrations of Pt that were sequestered in the chorion of the larval compared with the zebrafish tissue. These results indicate that this method can be applied to quantitate the metal dose present in larval tissues. Further, this method may be adjusted to identify specific metals or metal-based compounds in a broad range of exposure and dosing studies.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9281581PMC
http://dx.doi.org/10.3791/63587DOI Listing

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