AI Article Synopsis
- A new method for detecting senescent cells uses lipofuscin labeling with a fluorescent reporter via a biorthogonal chemical reaction.
- The process involves first interacting lipofuscin with a specific Sudan Black B derivative (SBB-N) and then reacting it with a fluorophore (BODIPY) to visualize senescent cells.
- The technique was tested on various senescent cell types, showing clear fluorescence in senescent cells compared to proliferative ones, confirming the method's effectiveness.
Article Abstract
A new method for senescent cell detection is described, which is based on lipofuscin labeling with a fluorescent reporter through a biorthogonal strain-promoted azide-alkyne cycloaddition. The sensing protocol involves a first step where the interaction of lipofuscin with a Sudan Black B derivative containing an azide moiety (SBB-N ) is carried out. In the final step, the azide moiety reacts with a fluorophore containing a cyclooctene ring (BODIPY). The efficacy of this two-step protocol is assessed in senescent melanoma SK-MEL-103 cells, senescent triple-negative breast cancer MDA-MB-231 cells and senescent WI-38 fibroblasts. In all cases, a clear fluorescence pattern was observed in senescent cells, compared to proliferative cells, only when the SBB-N -BODIPY probe was formed. Our results provide an alternative tool for the detection of senescent cells, based on an in situ bio-orthogonal reaction for lipofuscin labeling.
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| http://dx.doi.org/10.1111/febs.16477 | DOI Listing |
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