Protein microarrays are useful tools for detecting the presence of a target where different prey and bait combinations exist. Here we describe the extended application for a functional target-oriented screening assay with full length Heat shock proteins (HSPs ) for the identification of novel compounds.
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Alzheimers Dement
December 2024
University of Wisconsin School of Medicine and Public Health, Madison, WI, USA.
Background: DNA microarray-based studies report differentially methylated positions (DMPs) in blood between cognitively unimpaired persons (CU) and persons with late-onset dementia due to Alzheimer's disease (AD) or Mild Cognitive Impairment (MCI) but interrogate less than 4% of the human genome. Whole genome methylation sequencing (WGMS) quantifies DNA methylation levels across the entire human genome (>25 million CpG loci). Using WGMS, we previously reported 28,038 DMPs within 2,707 genes between persons with and without AD.
View Article and Find Full Text PDFGenetic Diagnostics and Phenotypic Profiling of a Girl With Autosomal Recessive Intellectual Developmental Disorder and Autism.
Cureus
November 2024
Laboratory of Genomic Medicine, GHC Genetics SK Ltd. Science Park, Comenius University in Bratislava, Bratislava, SVK.
In this article, we present a case study of a five-year-old girl with autism and developmental delay, conducted at the Academic Center for Autism Research in Bratislava, Slovakia. The girl was diagnosed using Autism Diagnostic Observation Schedule-Second Edition (ADOS-2) and Autism Diagnostic Interview-Revised (ADI-R) instruments and met the criteria for autism spectrum disorder. Intellectual functioning was in the markedly below-average range, as indicated by the Snijders-Oomen Nonverbal Intelligence Test-Revised (SON-R) examination, and her level of adaptive functioning was significantly reduced.
View Article and Find Full Text PDFIdentification of gastric cancer biomarkers through analysis of microarray based datasets.
Biochem Biophys Rep
December 2024
Department of Biochemistry & Molecular Biology, Institute of Biochemistry, Biotechnology and Bioinformatics (IBBB), The Islamia University of Bahawalpur, Bahawalpur, (63100), Pakistan.
Gastric cancer is among the most prevalent cancers worldwide including in Pakistan. Late diagnosis of gastric cancer leads to reduced survival. The present study aimed to investigate biomarkers for early diagnosis and prognosis of gastric cancer.
View Article and Find Full Text PDFHydrogel-Based Microdroplet Ensembles Encapsulating Multiplexed EXPAR Assays for Trichromic Digital Profiling of MicroRNAs and in-Depth Classification of Primary Urethral Cancers.
Nano Lett
December 2024
Institute for Advanced Study, Shenzhen University, Shenzhen, Guangdong 518060, People's Republic of China.
The primary challenge in microarray-based biological analysis lies in achieving the sensitive and specific detection of single-molecule targets while ensuring high reproducibility. A user-friendly digital imaging platform has been developed for the encoded trichromic profiling of circulating microRNAs (miRNAs). This platform replaces the traditional exponential polymerase amplification reaction (EXPAR) conducted on the microliter scale with a system that confines the amplification process within thousands of femtoliter-sized microdroplet reactors, cross-linked from tetra-armed poly(ethylene glycol) acrylate (Tetra-PEGA) and poly(ethylene glycol) dithiol (HS-PEG-SH), thus offering significant advantages, including minimal sample input, enhanced reactivity, and simplified analytical procedures.
View Article and Find Full Text PDFA microarray-based IgE-molecular mimicry index (IgE-MMI): A biomarker for disease severity, clinical phenotypes, and therapeutic response in atopic dermatitis?
Allergy
December 2024
Department of Pediatric Respiratory Medicine, Immunology and Critical Care Medicine, Charité-Universitätsmedizin Berlin, a corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, Germany.
Background: The role of autoimmune IgE responses in atopic dermatitis (AD) is highly debated. While IgE targeting self-proteins has been extensively studied, IgE responses induced by human-homologous exogenous molecular allergens (HEMAs) remains less understood.
Aim: To investigate whether IgE antibody responses to HEMAs are associated with AD, its severity, and response to dupilumab.
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