Severity: Warning
Message: file_get_contents(https://...@pubfacts.com&api_key=b8daa3ad693db53b1410957c26c9a51b4908&a=1): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests
Filename: helpers/my_audit_helper.php
Line Number: 176
Backtrace:
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 176
Function: file_get_contents
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 250
Function: simplexml_load_file_from_url
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 1034
Function: getPubMedXML
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3152
Function: GetPubMedArticleOutput_2016
File: /var/www/html/application/controllers/Detail.php
Line: 575
Function: pubMedSearch_Global
File: /var/www/html/application/controllers/Detail.php
Line: 489
Function: pubMedGetRelatedKeyword
File: /var/www/html/index.php
Line: 316
Function: require_once
A polyphasic approach was used to describe strain RB6PN24, a novel actinobacterium isolated from peat swamp forest soil collected from Rayong province, Thailand. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain belonged to the genus and showed the highest sequence similarities to IFO 15206 (98.7 %) and MMS16-CNU292 (98.5 %). Strain RB6PN24 contained major amounts of -diaminopimelic acid, galactose, mannose and ribose in the whole-cell hydrolysates. MK-9(H) and MK-9(H) were the predominant menaquinones of the micro-organism. The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, an unidentified lipid, four unidentified aminolipids and six unidentified phospholipids. Mycolic acids were not present. The major fatty acids were iso-C, iso-C, anteiso-C, iso-C, anteiso-C and C. The draft genome size of strain RB6PN24 was 8.09 Mbp, with 72.1 mol% G+C content and predicted to contain at least 44 biosynthetic gene clusters encoding diverse secondary metabolites. Furthermore, the strain exhibited low average nucleotide identity and digital DNA-DNA hybridization values with MMS16-CNU292 (89.1 %, 42.4 %) and DSM 41654 (79.5 %, 25.5 %). The results of phenotypic, chemotaxonomic, genotypic and phylogenetic analyses revealed that strain RB6PN24 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RB6PN24 (=TBRC 14818=NBRC 115116). In addition, the comparison of the whole genome sequences and phenotypic features suggested that and belong to the same species. Therefore, it is proposed that is reclassified as a later heterotypic synonym of .
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Source |
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http://dx.doi.org/10.1099/ijsem.0.005356 | DOI Listing |
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