In this study, a colloidal gold immunochromatographic strip was developed for simultaneous detection of rhein and aloe-emodin in rhubarb. The cutoff value, defined as the lowest concentration for which the test line was invisible on the strip, was 50 ng mL for both rhein and aloe-emodin. By contrast, the cutoff value for emodin was 2,000 ng mL. No competitive inhibition was observed up to 5,000 ng mL of physcion, chrysophanol, sennoside A, sennoside B, or rhaponticin. Semiquantitative analyses of the total contents of rhein and aloe-emodin in raw drug materials via our colloidal gold immunochromatographic strips produced results agreeable with those determined by HPLC. Taken together, our findings suggest that the implementation of our colloidal gold immunochromatographic strips provides a rapid one-step method for estimating the total contents of rhein and aloe-emodin, which may represent a powerful tool for quality control of rhubarb.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9064498 | PMC |
| http://dx.doi.org/10.1155/2022/7067245 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Potential Effects of Bioactive Compounds of Plant-Based Foods and Medicinal Plants in Chronic Kidney Disease and Dialysis: A Systematic Review.
Nutrients
December 2024
Department of Pharmaceutical & Health Sciences, School of Pharmacy, Universidad San Pablo-CEU, CEU Universities, Urbanización Montepríncipe, 28660 Madrid, Spain.
Background: The bioactive components of plant foods and medicinal plants have attracted interest due to their potential impact on the progression of chronic kidney disease (CKD) and outcomes.
Objective: This study aimed to conduct a critical and quantitative systematic review of randomized clinical trials (RCTs) investigating the potential effects of selected phytochemicals from plant-based foods and medicinal plants in CKD and dialysis patients.
Methods: The review included studies that related plant-based bioactive compounds (curcumin, propolis, sulforaphane, betalain, catechins, rhein, emodin, aloe-emodin, flavonoids, and triptolide) and medicinal plants (green tea, rhubarb, , and Hook F) in CKD and dialysis patients.
The Activity of 1,8-Dihydroanthraquinone Derivatives in Nervous System Cancers.
Molecules
December 2024
Department of Biochemistry and Molecular Biology, Medical University of Lublin, 1 Chodzki Str., 20-093 Lublin, Poland.
Primary and metastatic tumors of the nervous system represent a diverse group of neoplasms, each characterized by distinct biological features, prognostic outcomes, and therapeutic approaches. Due to their molecular complexity and heterogeneity, nervous system cancers (NSCs) pose significant clinical challenges. For decades, plants and their natural products with established anticancer properties have played a pivotal role in the treatment of various medical conditions, including cancers.
View Article and Find Full Text PDFResearch Progress on the Anti-Liver Cancer Mechanism and Toxicity of Rhubarb Anthraquinone.
Drug Des Devel Ther
December 2024
Department of Pharmacy, Chengdu Integrative TCM & Western Medicine Hospital, Chengdu, Sichuan, 610095, People's Republic of China.
Ethnopharmacological Relevance: Rhubarb has the effect of breaking blood stasis and abnormal mass, and was often used to treat various tumor diseases including liver cancer in ancient China. Recipes containing rhubarb have anti-liver cancer properties and are still used today. However, the main components and mechanism of action of rhubarb against liver cancer are still unclear.
View Article and Find Full Text PDFTreatment of constipation with and its compatibility prescriptions.
Chin Herb Med
October 2024
The Second Hospital of Shandong University, Jinan 250033, China.
Constipation is a common and prevalent digestive system disease in clinical practice, which seriously affects human physical and mental health. Currently, chemical drugs have good short-term therapeutic effects. However, because of their adverse reactions, easy recurrence after drug discontinuation, and dependence with long-term use, the long-term efficacy is unsatisfactory.
View Article and Find Full Text PDFRapid Identification of Chemical Constituents of Sanhua Decoction in Vivo and in Vitro and the Metabolites of Rhubarb Anthraquinone Aglycone by Ultra-High Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Tandem Mass Spectrometry.
J Sep Sci
November 2024
School of Integrated Traditional Chinese and Western Medicine, Hebei University of Chinese Medicine, Shijiazhuang, P. R. China.
Sanhua Decoction (SHD) is a classic prescription for the treatment of stroke in the clinic. Based on the combination strategy in vitro and in vivo, the chemical constituents of SHD were characterized by ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry, and the metabolites of five effective anthraquinone aglycones (emodin, chrysophanol, rhein, aloe-emodin, and physcion) of rhubarb in SHD were studied. A total of 222 compounds were isolated and identified in vitro, including 50 flavonoids and their glycosides, 41 coumarins, 22 anthraquinones, 22 tannins, 14 phenylpropanoids, 16 alkaloids, 18 organic acids and their esters, 14 lignans, six anthrones, and 19 other compounds.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!