AI Article Synopsis
- Metal-organic frameworks (MOFs), specifically zeolitic imidazolate framework-8 (ZIF8), are explored for creating a new composite microcarrier (MC) to support human mesenchymal stem cells (hMSCs) adhesion and growth due to their high porosity and tunable features.
- The ZIF8 MCs were developed by combining polydopamine and polyethyleneimine on polystyrene beads, and various techniques were used to analyze their chemical properties and stability.
- Tests showed that ZIF8 MCs provided an excellent environment for human adipose-derived mesenchymal stem cells (hADSCs) to adhere and grow, indicating their potential as cost-effective tools for promoting cell culture in tissue engineering
Article Abstract
Metal-organic frameworks (MOFs) have high porosity, large surface area, and tunable functionality and have been widely used for drug loading. The aim of this study was to exploit unique features of zeolitic imidazolate framework-8 (ZIF8) to develop an innovative composite microcarrier (MC) for human mesenchymal stem cells (hMSCs) adhesion and proliferation. ZIF8 MCs were prepared by immobilization of polydopamine/polyethyleneimine (PDA/PEI) and ZIF8 on the surface of polystyrene beads. The chemical properties of MCs such as coating stability and homogeneity were characterized by different techniques such as ATR-FTIR, XRD, EDX, SEM, and contact angle. The prepared MCs were tested using human adipose-derived mesenchymal stem cells (hADSCs) in both static and dynamic conditions, and results were compared to a commercially available MC (Star-Plus), polydopamine coated MCs and amine-functionalized MCs as a control. Results show that PDA/PEI/ZIF8 coated MCs (in short: ZIF8 MCs) provides an excellent biocompatible environment for hADSCs adhesion and growth. In conclusion, ZIF8 MCs represent suitable and low-cost support for hADSCs culture and expansion, which can maximize cell yield and viability while preserving hADSCs multipotency. The present findings have revealed this strategy has the potential for chemical and topographical modification of MCs in tissue engineering applications.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9054200 | PMC |
| http://dx.doi.org/10.1039/d0ra04090h | DOI Listing |
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