AI Article Synopsis
- A new fluorescent probe named IN-CZ has been developed for detecting sulfite, utilizing a mechanism based on intramolecular charge transfer.
- The probe works through a chemical reaction with sulfite, boasting a detection limit of 2.99 × 10 M and providing quick results within a minute.
- IN-CZ offers high selectivity for sulfite over other reactive species and can be used for ratiometric fluorescence imaging in living cells, specifically targeting changes in sulfite levels in mitochondria.
Article Abstract
A real-time ratiometric fluorescent probe (IN-CZ) for highly selective detection of sulfite was designed and synthesized, which is based on modulating the intramolecular charge transfer (ICT) of the hemicyanine dye platform. The mechanism of using the probe is mainly through the Michael addition that occurs between IN-CZ and sulfite with a detection limit of 2.99 × 10 M. IN-CZ displays a fast response (within 1 minute) and is highly selective for SO /HSO over ROS, biologically relevant ions, biological mercaptans and other reactive species. More importantly, IN-CZ was suitable for ratiometric fluorescence imaging in living cells, by real-time monitoring of SO /HSO changes in mitochondria targeted in living cells.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9066615 | PMC |
| http://dx.doi.org/10.1039/c9ra03207j | DOI Listing |
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