Exploring the mode of action of inhibitors targeting the PhoP response regulator of through comprehensive pharmacophore approaches.

The PhoQ/PhoP two-component system regulates the physiological and virulence functions of . However, the mode of action of known PhoP inhibitors is unclear. We systematically constructed a pharmacophore model of inhibitors to probe the interface pharmacophore model of the PhoP dimer, coupling it with Ligplot analysis. We found that these inhibitors bind on the α5-helix, altering the conformation and interfering with PhoP binding on DNA.