AI Article Synopsis
- A thrombin aptasensor was developed that detects the presence of thrombin by changing the aptamer's shape when it binds to the target, which increases fluorescence due to the closeness of DNA-templated copper/silver nanoclusters.
- Two DNA templates were engineered to include segments for copper/silver nanoclusters and an aptamer, allowing for a sensitive detection mechanism as they come together upon target binding.
- This innovative sensor demonstrated the ability to detect thrombin as low as 1.6 nM in a selective manner and was successfully tested in real fetal bovine serum samples.
Article Abstract
A highly sensitive thrombin aptasensor was constructed based on the alteration of the aptamer conformation induced by the target recognition and the turn-on fluorescence due to the proximity of two darkish DNA-templated copper/silver nanoclusters (DNA-Cu/Ag NCs). Two DNA templates were designed as the functional structures consisting of the Cu/Ag NC-nucleation segment located at two termini or one terminus and the aptamer segment in the middle of a DNA template. Two darkish DNA-Cu/Ag NCs came close to each other when the aptamer combined with the target due to the conformational alteration of the aptamer structure, resulting in an increased fluorescence signal readout. Thrombin was sensitively determined as low as 1.6 nM in the range of 1.6-8.0 nM with a high selectivity. Finally, this sensor succeeded in detecting thrombin in a real fetal bovine serum.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9056932 | PMC |
| http://dx.doi.org/10.1039/d0ra04609d | DOI Listing |
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