Activated sludge from the A/O process in a wastewater treatment plant (WWTP) was used as the seed sludge for enrichment to achieve faster growth of nitrifying bacteria and higher nitrification efficiency of the filler made by nitrifying bacteria. The bacterial community was enriched in a self-circulating bacteria culture tank by a continuous ammonia feeding mode. The study found that the nitrifying bacteria community was enriched in 38 days with the ammonia oxidation rate of approximately 275.58 mg (L h). High-throughput sequencing demonstrated that belonging to ammonia-oxidizing bacteria (AOB) was predominant in the sludge after 38 days at a ratio extending from 0.43% to 61.91%. The enriched sludge was used as the bacterial source and the immobilization was carried out with polyvinyl alcohol (PVA). After the recovery culture, the ammonia oxidation rate of the filler was up to 44.61 mg (L h) for the treatment of municipal wastewater, and the effluent ammonia was below 1 mg L, indicating that the immobilized filler is effective for municipal wastewater nitrification. Scanning electron microscope (SEM) observations showed that immobilized fillers were highly porous and bacteria adhered to the network structure, demonstrating that the filler provided a good growth microenvironment for microorganisms.
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http://dx.doi.org/10.1039/d0ra01498b | DOI Listing |
Bioresour Technol
January 2025
Collaborative Innovation Center of Atmospheric Environment and Equipment Technology, Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control, School of Environmental Science and Engineering, Nanjing University of Information Science & Technology, Nanjing 210044 China. Electronic address:
Iron-carbon (Fe-C) based biofilters have shown significant advantages in treating mariculture wastewater by facilitating the mixotrophic heterotrophic nitrification-aerobic denitrification (HNAD) process. However, the effects of Fe-C materials and varying carbon-to-nitrogen (C/N) ratios on N removal and C reduction performance remain insufficiently explored. This study demonstrated that the Fe-C biofilter (R-Fe) achieved significantly higher NO-N removal efficiency (65.
View Article and Find Full Text PDFAppl Microbiol Biotechnol
January 2025
Department of Biotechnology, Universidad Autónoma Metropolitana-Iztapalapa, Av. Ferrocarril San Rafael Atlixco 186, Col. Leyes de Reforma 1A Sección, Iztapalapa, CDMX, Mexico City, Mexico.
The relationship of microbial community and cometabolic consumption of 2-chlorophenol (2-CP) in a nitrifying sequencing batch reactor (SBR) was studied. The assessment of the population dynamics of the nitrifying sludge during the cometabolic 2-CP consumption with increasing ammonium (NH) concentrations in the SBR showed the presence of 39 different species of which 10 were always present in all cycles. Fifty-five percent of the species found were grouped as Proteobacteria (45% as β-proteobacteria and 10% as γ-proteobacteria class), 30% as Acidobacteria, and 15% as Deinococcus-Thermus phyla.
View Article and Find Full Text PDFWorld J Microbiol Biotechnol
January 2025
Key Laboratory for Humid Subtropical Eco-Geographical Processes of the Ministry of Education, School of Geographical Sciences, Fujian Normal University, Fuzhou, China.
Nitrification, the oxidation of ammonium to nitrate via nitrite, links nitrogen fixation and nitrogen loss processes, playing key roles in coastal nitrogen cycle. However, few studies have simultaneously examined both ammonia-oxidizing and nitrite-oxidizing microbes. This work investigated the abundance and community structure of ammonia-oxidizing archaea (AOA), ammonia-oxidizing bacteria (AOB), and nitrite-oxidizing bacteria (NOB) using archaeal amoA gene, bacterial amoA gene, and NOB nxrB gene, respectively, through q-PCR and Sanger sequencing along the Changjiang Estuary salinity gradient.
View Article and Find Full Text PDFWater Res
December 2024
Eawag: Swiss Federal Institute of Aquatic Science and Technology, Überlandstrasse 133, 8600, Dübendorf, Switzerland. Electronic address:
Aerobic granular sludge (AGS) is usually considered to be a biofilm system consisting of granules only, although practical experience suggests that flocs and granules of various sizes co-exist. This study thus focused on understanding the contribution of flocs and granules of various sizes to nitrification in a full-scale AGS-based wastewater treatment plant (WWTP) operated as a sequencing batch reactor (SBR). The size distribution in terms of total suspended solids (TSS) and the distribution of the nitrifying communities and activities were monitored over 14 months.
View Article and Find Full Text PDFWaste Manag
January 2025
Guangxi Bossco Environmental Protection Technology Co., Ltd., Nanning 530007, China.
Ammonia nitrogen (NH-N) discharge has caused eutrophication of water bodies and harm to humans and organisms. In this work, polyvinyl alcohol (PVA), sodium carboxymethyl cellulose (CMC), diatomite (DE), and FeO were used to prepare magnetic immobilized carriers by encapsulating microorganisms for the treatment of NH-N wastewater. The response surface methodology was used to explore the optimal ratio of the immobilized carriers.
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