Electrically-driven handling of gametes and embryos: taking a step towards the future of ARTs.

Lab Chip

Centre for Microsystems Technology (CMST), Imec and Ghent University, Technologiepark Zwijnaarde 126, 9052 Zwijnaarde, Ghent, Belgium.

Published: May 2022

Electrical stimulation of gametes and embryos and on-chip manipulation of microdroplets of culture medium serve as promising tools for assisted reproductive technologies (ARTs). Thus far, dielectrophoresis (DEP), electrorotation (ER) and electrowetting on dielectric (EWOD) proved compatible with most laboratory procedures offered by ARTs. Positioning, entrapment and selection of reproductive cells can be achieved with DEP and ER, while EWOD provides the dynamic microenvironment of a developing embryo to better mimic the functions of the oviduct. Furthermore, these techniques are applicable for the assessment of the developmental competence of a mammalian embryo . Such research paves the way towards the amelioration and full automation of the assisted reproduction methods. This article aims to provide a summary on the recent developments regarding electrically stimulated lab-on-chip devices and their application for the manipulation of gametes and embryos .

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1lc01160jDOI Listing

Publication Analysis

Top Keywords

gametes embryos
12
electrically-driven handling
4
handling gametes
4
embryos step
4
step future
4
future arts
4
arts electrical
4
electrical stimulation
4
stimulation gametes
4
embryos on-chip
4

Similar Publications

A tale of two tissues: Patterning of the epidermis through morphogens and their role in establishing tracheal system organization.

Cells Dev

January 2025

Departamento de Biología Celular y Fisiología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de Mexico, Mexico. Electronic address:

Throughout embryonic development, cells respond to a diverse set of signals and forces, making individual or collective decisions that drive the formation of specialized tissues. The development of these structures is tightly regulated in space and time. In recent years, the possibility that neighboring tissues influence one another's morphogenesis has been explored, as some of them develop simultaneously.

View Article and Find Full Text PDF

Why has the 'miracidium' of Notocotylidae (Trematoda: Digenea) lost all stage-specific traits?

Parasitol Res

January 2025

Department of Invertebrate Zoology, Saint Petersburg University, Universitetskaya emb., 7/9, Saint Petersburg, 199034, Russia.

Digeneans of the family Notocotylidae differ from other digeneans in their peculiar eggs. The eggs feature a pair of long filaments extending from their poles, and their contents differ significantly from what we expect to observe in the eggs of digeneans. Instead of a ciliated miracidium larva, the notocotylid egg contains a tiny few-celled mother sporocyst.

View Article and Find Full Text PDF

The quality and quantity of stripped and testicular wild northern pike (Esox lucius) sperm was compared and the effectiveness of short-term storage was assessed. Stripped sperm (SS) was collected using abdominal massage. Next, the fish were decapitated and the testes were removed.

View Article and Find Full Text PDF

Effect of Time-Lapse Incubation System on In Vitro Development of Alpaca Embryos.

Reprod Domest Anim

February 2025

Veterinary Embryology Laboratory, Professional School of Veterinary Medicine, Universidad Nacional de San Antonio Abad del Cusco, Sicuani-Cusco, Peru.

Currently, incubators with a time-lapse system are widely used for in vitro embryo production in several species, however, their effect on alpaca embryo development compared to conventional incubators remains unknown. The aim of this study was to compare early in vitro embryo development in alpacas using a time-lapse incubator system versus a conventional incubator. Ovaries were obtained from a slaughterhouse and 1048 cumulus-oocyte complexes (COCs) were collected and in vitro matured for 26 h in either a time-lapse system (n = 542) or a conventional incubator (n = 542).

View Article and Find Full Text PDF

Generation of bovine decellularized testicular bio-scaffolds as a 3D platform for testis bioengineering.

Front Bioeng Biotechnol

January 2025

Department of Agricultural and Environmental Sciences - Production, Landscape, Agroenergy, Università degli Studi di Milano, Milan, Italy.

Accelerating the genetic selection to obtain animals more resilient to climate changes, and with a lower environmental impact, would greatly benefit by a substantial shortening of the generation interval. One way to achieve this goal is to generate male gametes directly from embryos. However, spermatogenesis is a complex biological process that, at present, can be partially reproduced only in the mouse.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!