AI Article Synopsis
- The manuscript explores using geometry to enhance tile-based DNA self-assembly in two and three dimensions, adding to traditional sequence-based methods.
- Successful DNA crystal assembly depends on both the complementary sticky-end sequences and the geometric arrangement of DNA motifs, which prevents hindrance during assembly.
- By adjusting the length of motif branches, researchers can program DNA motifs to self-sort or mix, leading to the creation of various types of crystals and improved versatility in DNA self-assembly.
Article Abstract
This manuscript introduces geometry as a means to program the tile-based DNA self-assembly in two and three dimensions. This strategy complements the sequence-focused programmable assembly. DNA crystal assembly critically relies on intermotif, sticky-end cohesion, which requires complementarity not only in sequence but also in geometry. For DNA motifs to assemble into crystals, they must be associated with each other in the proper geometry and orientation to ensure that geometric hindrance does not prevent sticky ends from associating. For DNA motifs with exactly the same pair of sticky-end sequences, by adjusting the length (thus, helical twisting phase) of the motif branches, it is possible to program the assembly of these distinct motifs to either mix with one another, to self-sort and consequently separate from one another, or to be alternatingly arranged. We demonstrate the ability to program homogeneous crystals, DNA "alloy" crystals, and definable grain boundaries through self-assembly. We believe that the integration of this strategy and conventional sequence-focused assembly strategy could further expand the programming versatility of DNA self-assembly.
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| http://dx.doi.org/10.1021/jacs.2c02456 | DOI Listing |
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