Biocorrosion of pure and SLA titanium surfaces in the presence of and its effects on osteoblast behavior.

: The study aims to investigate the biocorrosion behavior of on pure and SLA titanium surfaces and its effects on surface characteristics and osteoblast behavior. : Pure and SLA titanium specimens were immersed in culture medium with and incubated for 7 days. colonization on the pure and SLA titanium surfaces was observed by scanning electron microscopy (SEM). The pure and SLA titanium surface characteristics were analyzed X-ray photoelectron spectroscopy (XPS), surface roughness and surface wettability. The corrosion behaviors of pure and SLA titanium specimens were evaluated by electrochemical corrosion test. The osteoblast behavior of MC3T3-E1 cells on the pure and SLA titanium surfaces after colonization was investigated by cell adhesion and western blot assays. : colonized on the pure and SLA titanium surfaces was observed by SEM. The XPS analysis demonstrated reductions in the relative levels of titanium and oxygen and obvious reductions of dominant titanium dioxide (TiO) on both titanium surfaces after immersing the metal in culture. In addition, their roughness and wettability were changed. Correspondingly, the electrochemical corrosion test results revealed significant decreases in the corrosion resistance and increases in the corrosion rate of the pure and SLA titanium specimens after immersion in culture. The results of the study showed that the pre-corroded pure and SLA titanium surfaces by exhibited lower osteocompatibility and down-regulated the adhesion, spreading and osteogenic differentiation abilities of MC3T3-E1 cells. : was able to colonize on the pure and SLA titanium surfaces and weaken their surface properties, especially a decrease in the protective TiO film, which induced the biocorrosion and further negatively affected the osteoblast behavior.