Tissue engineering has emerged as a promising approach for soft tissue regeneration. Three-dimensional (3D) cell printing showed great potential for producing cell-encapsulated scaffolds to repair tissue defects. The advantage of 3D cell printing technology is precise cell loading in scaffolds to achieve tissue regeneration instead of only relying on the cells from surrounding tissue or blood. A new acellular dermal matrix/gelatin-sodium alginate (ADM/A/G) scaffold with living gingival fibroblasts was constructed by 3D cell printing technology for potential oral soft tissue regeneration in this study, and the biological characteristics of the 3D cell printing scaffolds were evaluated. The residue of nucleic acid and growth factors in ADM were detected. Three biomaterials were mixed at an appropriate radio with human gingival fibroblasts (hGFs) to prepare bioinks. Two kinds of layer scaffolds were fabricated by 3D cell printing technology. The mechanical strength and degradability of the scaffolds were determined by measuring their compressive modulus and mass loss. CCK-8 assay and calcein-AM/PI staining were conducted to detect the cell proliferation and viability in 3D cell printing scaffolds. The morphology of the hGFs in the scaffolds were observed using SEM and FITC-phalloidin staining. The expression of COL1A1, PECAM1, and VEGF-A of hGFs in the scaffolds were quantified by qRT-PCR. The gelatin-sodium alginate (A/G) scaffolds were used as control group in all experiments. Compared with the control group, 3D cell printing ADM/A/G scaffolds showed better mechanical strength and longer degradation time. The ADM/A/G scaffolds obviously had a better promotion effect on cell proliferation and viability. Most of the hGFs observed had a fully extended spindle morphology in the ADM/A/G scaffolds but oval morphology in the control group. The expression of COL1A1 was significantly higher than in the control group with time, and the expression of PECAM1 and VEGF-A was slightly higher in ADM/A/G scaffolds on day 14. 3D cell printing gingival fibroblast-ADM/A/G scaffolds showed excellent biological properties, which could be potentially useful in oral soft tissue regeneration.
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http://dx.doi.org/10.1039/d0ra02082f | DOI Listing |
PLoS One
January 2025
The Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, Maryland, United States of America.
The extrusion bioprinting of collagen material has many applications relevant to tissue engineering and regenerative medicine. Freeform Reversible Embedding of Suspended Hydrogels (FRESH) technology is capable of 3D printing collagen material with the specifications and details needed for precise tissue guidance, a crucial requirement for effective tissue repair. While FRESH has shown repeated success and reliability for extrusion printing, the mechanical properties of completed collagen prints can be improved further by post-print crosslinking methodologies.
View Article and Find Full Text PDFAdv Sci (Weinh)
January 2025
State Key Laboratory for Manufacturing Systems Engineering, Xi'an Jiaotong University, Xi'an, 710049, P. R. China.
Micro/nanoscale 3D bioelectrodes gain increasing interest for electrophysiological recording of electroactive cells. Although 3D printing has shown promise to flexibly fabricate 3D bioelectronics compared with conventional microfabrication, relatively-low resolution limits the printed bioelectrode for high-quality signal monitoring. Here, a novel multi-material electrohydrodynamic printing (EHDP) strategy is proposed to fabricate bioelectronics with sub-microscale 3D gold pillars for in vitro electrophysiological recordings.
View Article and Find Full Text PDFAdv Sci (Weinh)
January 2025
ETH Zurich, Department of Biosystems Science and Engineering, Klingelbergstrasse 48, Basel, CH-4056, Switzerland.
Neo-vascularization plays a key role in achieving long-term viability of engineered cells contained in medical implants used in precision medicine. Moreover, strategies to promote neo-vascularization around medical implants may also be useful to promote the healing of deep wounds. In this context, a biocompatible, electroconductive borophene-poly(ε-caprolactone) (PCL) 3D platform is developed, which is called VOLT, to support designer cells engineered with a direct-current (DC) voltage-controlled gene circuit that drives secretion of vascular endothelial growth factor A (VEGFA).
View Article and Find Full Text PDFSTAR Protoc
January 2025
Department of Medicine, Division of Hematology, Oncology, and Transplantation, University of Minnesota Medical School, Minneapolis, MN, USA; Masonic Cancer Center, University of Minnesota Medical School, Minneapolis, MN, USA. Electronic address:
Tumor Treating Fields (TTFields) are electric fields clinically approved for cancer treatment, delivered via arrays attached to the patient's skin. Here, we present a protocol for applying TTFields to torso orthotopic and subcutaneous mouse tumor models using the inovivo system. We guide users on proper system component connections, study protocol design, mouse fur depilation, array application, and treatment condition adjustment and monitoring.
View Article and Find Full Text PDFAngew Chem Int Ed Engl
December 2024
Key Laboratory of Green Printing, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.
Self-assembled monolayers (SAM) as hole transport layers have been widely used in high-efficiency inverted perovskite solar cells (PSCs) exceeded 26 %. However, the poor coverage and non-uniform distribution on the substrate of SAM further restrict the improvement of device performance. Herein, we utilize the mixed SAM strategy via the MeO-2PACz along with perfluorotripropylamine (FC-3283) to improve the SAM coverage, aiming to accelerate the carrier transport, promote the perovskite growth, regulate the surface energy levels and suppress the nonradiative recombination.
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