AI Article Synopsis
- Eosinophils, important immune cells, are categorized into distinct subpopulations in mice based on their surface markers, but there’s limited research on these distinctions in humans, particularly in eosinophilic asthma patients.
- The study focused on analyzing eosinophil subpopulations in the peripheral blood and nasal polyp tissue of patients with severe eosinophilic asthma who also have chronic rhinosinusitis, comparing them to various control groups.
- Results revealed that severe eosinophilic asthma patients had a higher percentage of a specific eosinophil subtype (CD62L) in both blood and nasal tissues, indicating a unique accumulation pattern and functional differences in these cells between the two environments.
Article Abstract
Background: In mice models, eosinophils have been divided into different subpopulations with distinct phenotypes and functions, based on CD62L and CD101 patterns of membrane expression. Limited data are available in humans.
Objective: To investigate eosinophils subpopulations in peripheral blood (PB) and nasal polyp tissue (NP) from severe eosinophilic asthma (SEA) patients plus concomitant chronic rhinosinusitis with nasal polyps (CRSwNP).
Methods: We recruited 23 SEA patients (14 with CRSwNP); as controls, we enrolled 15 non-severe asthma patients, 15 allergic rhinitis patients without asthma and 15 healthy donors. Eosinophils were isolated from PB and NP and analysed by FACS. Eotaxin-3 and eotaxin-1 mRNA expression in NP tissue was also evaluated.
Results: A significantly higher percentage of circulating CD62L cells was observed in SEA, as compared with controls, expressing higher levels of CCR3, CD69 and lower levels of CD125 (IL-5R), CRTH2, CD86 and CD28 in comparison with CD62L cells. In NP, eosinophils showed a high proportion of CD62L phenotype, significantly greater than that observed in PB. Surface expression of IL-3R, IL-5R, CD69 and CD86 was significantly higher in CD62L eosinophils from NP than in those from blood. Moreover, eotaxin-3 mRNA expression positively correlated with the percentage of CD62L cells in NP.
Conclusion: Two different eosinophil subphenotypes can be identified in blood and NP of SEA patients, with a preferential accumulation of CD62L inflammatory cells in NP.
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| http://dx.doi.org/10.1111/cea.14153 | DOI Listing |
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