AI Article Synopsis
- The study aimed to assess the effectiveness of p16/Ki-67 dual-staining in identifying cervical intraepithelial neoplasia grade 2 or worse (CIN2+) among women with minor cervical abnormalities.
- Testing was conducted on 759 patients, evaluating cytology, high-risk HPV, and dual staining, with results showing that positivity rates for HR-HPV and dual staining increased with the severity of dysplasia.
- The dual-staining method demonstrated high sensitivity (97.3%) and an impressive specificity (99.3%), suggesting it could serve as an efficient tool in screening programs, potentially reducing unnecessary colposcopies.
Article Abstract
Background/aim: To evaluate p16/Ki-67 dual-staining performance for detection of cervical intraepithelial neoplasia grade 2 or worse (CIN2+) in the management of women with minor cervical abnormalities.
Patients And Methods: All 759 enrolled patients were tested for cytology, high-risk human papillomavirus (HR-HPV) and dual p16/Ki-67 staining.
Results: Positivity rates for HR-HPV and dual staining increased as dysplasia was worsened from non-CIN (37.6% and 0%) to CIN1 (62.5% and 1.6%) and CIN2+ (98.7% and 97.3%), respectively. HPV18 and HPV16 exhibited the highest odds ratios (53.16 and 11.31) in the CIN2+ group. Both p16/Ki-67 dual staining and HR-HPV presented similar sensitivities (97.3% and 98.7%, respectively) for CIN2+ detection. Dual staining specificity, however, was 99.3%, significantly higher compared to HR-HPV testing (52.2%). The utility of dual staining was evaluated in different screening strategies and appeared to reduce the number of colposcopies required for the detection of CIN2+ cases.
Conclusion: p16/Ki-67 dual-staining cytology is a surrogate triage biomarker in cytology-based screening programs, with high performance for efficient risk stratification of women with mild cervical abnormalities.
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| http://dx.doi.org/10.21873/anticanres.15738 | DOI Listing |
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