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SERS and Fluorescence-Active Multimodal Tessellated Scaffolds for Three-Dimensional Bioimaging. | LitMetric

SERS and Fluorescence-Active Multimodal Tessellated Scaffolds for Three-Dimensional Bioimaging.

ACS Appl Mater Interfaces

CIC biomaGUNE, Basque Research and Technology Alliance (BRTA), 20014 Donostia-San Sebastián, Spain.

Published: May 2022

AI Article Synopsis

  • The study discusses the growing importance of 3D cell models for researching bio-nano interactions and highlights the need for advanced imaging techniques for this purpose.
  • A novel method using surface-enhanced Raman scattering (SERS)-based microscopy is introduced, which enables real-time monitoring of cell proliferation in 3D environments using multifunctional 3D-printed scaffolds made from poly(lactic--glycolic acid) (PLGA).
  • By embedding fluorescent labels and SERS-active gold nanoparticles (AuNPs) in these scaffolds, researchers can simultaneously track cell growth and migration in 4D with minimal disturbance to the tissue model.

Article Abstract

With the ever-increasing use of 3D cell models toward studying bio-nano interactions and offering alternatives to traditional 2D and experiments, methods to image biological tissue in real time and with high spatial resolution have become a must. A suitable technique therefore is surface-enhanced Raman scattering (SERS)-based microscopy, which additionally features reduced photocytotoxicity and improved light penetration. However, optimization of imaging and postprocessing parameters is still required. Herein we present a method to monitor cell proliferation over time in 3D, using multifunctional 3D-printed scaffolds composed of biologically inert poly(lactic--glycolic acid) (PLGA) as the base material, in which fluorescent labels and SERS-active gold nanoparticles (AuNPs) can be embedded. The combination of imaging techniques allows optimization of SERS imaging parameters for cell monitoring. The scaffolds provide anchoring points for cell adhesion, so that cell growth can be observed in a suspended 3D matrix, with multiple reference points for confocal fluorescence and SERS imaging. By prelabeling cells with SERS-encoded AuNPs and fluorophores, cell proliferation and migration can be simultaneously monitored through confocal Raman and fluorescence microscopy. These scaffolds provide a simple method to follow cell dynamics in 4D, with minimal disturbance to the tissue model.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100500PMC
http://dx.doi.org/10.1021/acsami.2c02615DOI Listing

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