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Protocol for simultaneous detection of PRRS virus infection and dying cell populations by a flow cytometry double-staining approach.
STAR Protoc
March 2025
Department of Biology and Microbiology, South Dakota State University, Brookings, SD 57006, USA. Electronic address:
When investigating cellular responses to viral infections, there is a need to quantitatively distinguish between dying cells, infected cells, and cells that are both infected and dying. Here, we present a protocol to simultaneously detect porcine reproductive and respiratory syndrome virus (PRRSV) infection and cell death using a flow cytometry double-staining approach. We describe steps for cell seeding, infection, and collection; staining dead cells; sample fixation; and cell permeabilization.
View Article and Find Full Text PDFBeliefs and attitudes of family caregivers of People with dementia toward advance care planning: A Q methodology study.
Dementia (London)
March 2025
Georgia Baptist College of Nursing, Mercer University, USA.
Family caregivers' perspectives for advance care planning (ACP) are critically important to reflect the end-of-life wishes of people with dementia. Culture can influence ACP practice and people's perspectives toward it. Little is known about family caregivers' perspectives toward ACP in Asian countries, especially in Korea.
View Article and Find Full Text PDFUnveiling the cancer epidemic in India: a glimpse into GLOBOCAN 2022 and past patterns.
Lancet Reg Health Southeast Asia
March 2025
Division of Implementation Research, Indian Council of Medical Research (ICMR), Headquarters, New Delhi, 110029, India.
Background: The Global Cancer Observatory (GLOBOCAN) 2022 estimates highlight cancer as a significant global health problem. In the South-East Asia-region, India ranks third in terms of cancer incidence, second for mortality, and 121 in terms of crude rates globally. As cancer risks increase with age, particularly affecting older individuals, this study explores the current cancer burden in India by analyzing the cancer landscape across different age groups, determining the population's risk of cancer diagnosis and death, and projecting the future cancer burden aligned with past mortality patterns.
View Article and Find Full Text PDFCytotoxic chemotherapy in a 3D microfluidic device induces dendritic cell recruitment and trogocytosis of cancer cells.
Cancer Immunol Res
March 2025
Seoul National University, Seoul, Korea (South), Republic of.
Cytotoxic chemotherapy that kills cancer cells can also elicit anti-tumor immune responses. Therefore, understanding the immunogenic context of cytotoxic chemotherapy can improve combination immunotherapies. In this study, we sought to improve our understanding about dendritic cell (DC) dynamics in cytotoxic chemotherapy-treated tumor tissues by developing 3D microfluidic devices that enable high-resolution visualization of cellular dynamics.
View Article and Find Full Text PDFThe burden of COVID-19 based on disability-adjusted life years: a systematic review of available evidence.
Front Public Health
March 2025
Nephrology and Urology Research Center, Clinical Sciences Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Background: The present study tries to evaluate and summarize the available evidence to provide insights into the COVID-19 burden worldwide using disability-adjusted life years (DALYs) and compare the level of damage across countries during this pandemic.
Method: We conducted a systematic review following the preferred reporting items for systematic reviews and meta-analyses (PRISMA) guidelines to investigate the global burden of COVID-19. Studies were identified through searches conducted on Ovid Medline, Cochrane, Science Direct, Scopus, and PubMed databases as well as, the Google Scholar search engine.
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