The nanopore sensing technique is an emerging method of detecting single molecules, and extensive research has gone into various fields, including nanopore sequencing and other applications of single-molecule studies. Recently, several researchers have explored the specific ion effects in nanopore channels, enabling a unique understanding of the Hofmeister effect at the single-molecule level. Herein, the recent advances of using nanopore sensing techniques are reviewed to study the Hofmeister effect and the physicochemical mechanism of this process is attempted. The challenges and goals are also discussed for the future in this field.
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http://dx.doi.org/10.1002/smll.202200921 | DOI Listing |
Chem Sci
December 2024
School of Chemistry and Chemical Engineering, Shandong University Jinan 250100 China
The development of universal electrochemical sensing platforms with high sensitivity and specificity is of great significance for advancing practical disease diagnostic methods and devices. Exploring the structural properties of electrode materials and their interaction with biomolecules is essential to developing novel and distinctive analytical approaches. Here, we innovatively investigated the effect of DNA length and configuration on DNA molecule transfer into the nanostructure of a nanoporous gold (NPG) electrode.
View Article and Find Full Text PDFJ Agric Food Chem
January 2025
Faculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China.
Conquering surface fouling of sensors caused by nonspecific adsorption and accumulation of foulants in a food matrix is of significance in accurate food safety analysis. Herein, an antifouling electrochemical aptasensor based on a Y-shaped peptide and nanoporous gold (NPG) for aflatoxin B1 detection in milk, tofu, and rice flour was proposed. The self-designed Y-shaped peptide involves an anchoring segment (-C), a support structure (-PPPP-), and an antifouling domain with two branches (-EK(KSRE)DER-) inspired by two bioactive peptides.
View Article and Find Full Text PDFNanoscale
January 2025
Department of Physics, McGill University, 845 Sherbrooke West, Montréal, Canada.
Solid-state nanopores exhibit dynamically variable sizes influenced by buffer conditions and applied electric field. While dynamical pore behavior can complicate biomolecular sensing, it also offers opportunities for controlled, modification of pore size post-fabrication. In order to optimally harness solid-state pore dynamics for controlled growth, there is a need to systematically quantify pore growth dynamics and ideally develop quantitative models to describe pore growth.
View Article and Find Full Text PDFChem Commun (Camb)
January 2025
State Key Laboratory of Biogeology and Environmental Geology, Engineering Research Center of Nano-Geomaterials of Ministry of Education, Faculty of Materials Science and Chemistry, China University of Geosciences, Wuhan 430074, China.
In recent years, researchers have drawn inspiration from natural ion channels to develop various artificial nanopores/nanochannels, including solid-state and biological. Through imitating the precise selectivity and single molecule sensing exhibited by natural ion channels, nanopores/nanochannels have been widely used in many fields, such as analyte detection, gene sequencing and so on. In these applications, the surface functionalization of nanopores/nanochannels directly determines the effectiveness in quantitative analysis and single molecule detection.
View Article and Find Full Text PDFJ Am Chem Soc
January 2025
Molecular Sensing and Imaging Center, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, P. R. China.
Nanopore technology holds great potential for single-molecule identification. However, extracting meaningful features from ionic current signals and understanding the molecular mechanisms underlying the specific features remain unresolved. In this study, we uncovered a distinctive ionic current pattern in a K238Q aerolysin nanopore, characterized by transient spikes superimposed on two stable transition states.
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