AI Article Synopsis
- Temporal patterning in neural progenitors is a key evolutionary strategy for increasing diversity in neurons.
- Researchers developed a method called NanoDam to study how known temporal transcription factors relate to neuronal development in Drosophila.
- The study identified additional factors, Homeobrain and Scarecrow, that are important for defining specific temporal windows in neural development and have similar roles in the visual systems of other species.
Article Abstract
Temporal patterning of neural progenitors is an evolutionarily conserved strategy for generating neuronal diversity. Type II neural stem cells in the Drosophila central brain produce transit-amplifying intermediate neural progenitors (INPs) that exhibit temporal patterning. However, the known temporal factors cannot account for the neuronal diversity in the adult brain. To search for missing factors, we developed NanoDam, which enables rapid genome-wide profiling of endogenously tagged proteins in vivo with a single genetic cross. Mapping the targets of known temporal transcription factors with NanoDam revealed that Homeobrain and Scarecrow (ARX and NKX2.1 orthologs) are also temporal factors. We show that Homeobrain and Scarecrow define middle-aged and late INP temporal windows and play a role in cellular longevity. Strikingly, Homeobrain and Scarecrow have conserved functions as temporal factors in the developing visual system. NanoDam enables rapid cell-type-specific genome-wide profiling with temporal resolution and is easily adapted for use in higher organisms.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9616798 | PMC |
| http://dx.doi.org/10.1016/j.devcel.2022.04.008 | DOI Listing |
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