Simultaneous quantification of peroxidase/ascorbate in vegetables with slope and intercept of kinetic curves in a flow-injection recirculating-catalysis system.

In using a flow-injection recirculating-catalysis system developed by us to research the simultaneous quantification for peroxidase and ascorbate, it was discovered that the concentrations of peroxidase activity and ascorbate are correlative with the slope and the negative intercept of the linear response curve during a peroxidase-catalyzed kinetic course. Therefore, based on this finding, a new analytical method and a simplified equation for quantifying the peroxidase activity concentration were proposed, Then, test conditions were optimized, finally the use of the method has realized the simultaneous determination for peroxidase of 2-40 U/L and ascorbate of 0.4-12 mg/L in various vegetables (60 μL). The assayed results were consistent with the comparison method, in which the repeatability (RSD < 1.43%, n = 11) was satisfactory. Another important conclusion obtained in this study is that the determination of the peroxidase activity in biosamples must use the kinetic curve method for fear of the influence from the ascorbate's lag phase.