AI Article Synopsis
- - Yeast surface display is an advanced technique used to evolve and engineer proteins with specific traits.
- - The updated protocols focus on finding lead binders and enhancing their affinity through screening large libraries using magnetic beads and flow cytometry.
- - Once individual clones are identified, their binding affinities are further refined using mutagenesis and fluorescence-activated cell sorting in a repetitive cycle.
Article Abstract
Yeast surface display is a powerful directed evolution method for developing and engineering protein molecules to attain desired properties. Here, updated protocols are presented for purposes of identification of lead binders and their affinity maturation. Large libraries are screened by magnetic bead selections followed by flow cytometric selections. Upon identification and characterization of single clones, their affinities are improved by an iterative process of mutagenesis and fluorescence-activated cell sorting.
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| http://dx.doi.org/10.1007/978-1-0716-2285-8_2 | DOI Listing |
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