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Dual-signal portable microRNA biosensor based on a photothermal/visual strategy induced by cascading amplification techniques and horseradish peroxidase.

Talanta

January 2025

Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of the Ministry of Education, Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Materials Science, Hebei University, Baoding 071002, Hebei Province, PR China. Electronic address:

MicroRNAs (miRNAs) serve as potential biomarkers for many diseases such as cancer, neurodegenerative diseases and cardiovascular conditions. The portable and accurate detection of miRNA is of great significance for the early diagnosis, treatment optimization and prognostic evaluation of diseases. Herein, a photothermal/visual dual-mode assay for let-7a is developed utilizing oxidized 3, 3', 5, 5' - tetramethylbenzidine (oxTMB) as signal reporter.

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Temperature-sensitive driving assembled fluorescence hydrogel based dual-mode sensor for adsorbing and detecting of heavy metal cadmium ions in food and water.

Food Chem

December 2024

State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food Quality and Health of Tianjin, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China.

The denatured bovine serum albumin (dBSA) is coupled with the CdTe/CdS quantum dot and the resulting CdTe/CdS@dBSA complex is assembled and retained in the poly(n-isopropyl acrylamide) (PNIPAM) hydrogel via regulating temperature and pH to form the CdTe/CdS@dBSA-PNIPAM fluorescence hydrogel substrate, which is able to adsorb and sense cadmium ions (Cd). Based on this fluorescence hydrogel, a fluorescence and colorimetric dual-mode detection system is established to quantitatively detect Cd with a limit of detection (LOD) of 2.88 nM for fluorescence detection and 11.

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Multimodal nanoenzyme-linked aptamer assay for Salmonella typhimurium based on catalysis and photothermal effect of PB@Au.

Mikrochim Acta

January 2025

Shandong Provincial Key Laboratory of Animal Resistance Biology, Key Laboratory of Food Nutrition and Safety of Shandong, College of Life Science, Normal University, Shandong Normal University, Jinan, 250014, People's Republic of China.

A composite nanomaterial of Prussian blue@gold nanoparticles (PB@Au) with catalytic and photothermal properties was proposed, which combined with anti-matrix interference aptamers to achieve robust specificity and sensitivity in the detection of Salmonella typhimurium (S. typhimurium). The detection probe, PB@Au-Aptamer (PB@Au-Apt), was designed to exhibit high specificity for the target and catalyze the signal generation to produce a color change, thereby enabling rapid detection.

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Visual, sensitive, and selective detection of carcinogenic substances is highly desired in portable health protection and practical medicine production. However, achieving this goal presents significant challenges with the traditional single-mode sensors reported so far, as they have limited sensing mechanisms and provide only a single output signal. Here, we report an effective optical and electrical dual-mode sensor for the visual, sensitive, and selective detection of -nitrosodiethylamine (NDEA), a typical volatile carcinogenic substance, leveraging the synergy of ionic liquid-doped liquid crystals (IL-LC).

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A ratiometric fluorescence and visual sensor based on conjugated polymer nanoparticles@MnO probe for organophosphorus pesticides detection.

Talanta

December 2024

Key Laboratory of Medicinal Chemistry and Molecular Diagnosis (Hebei University), Ministry of Education, Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Materials Science, Hebei University, Baoding, 071002, Hebei, PR China. Electronic address:

Organophosphorus (OPs) pesticide residues pose significant threats to human health and the environment. To tackle this issue, we synthesized water-soluble fluorescent conjugated polymer nanoparticles (WSCPNs), which offer high fluorescence intensity, simple preparation methods, and ease of functionalization, making them ideal candidates for fluorescent sensing applications. These WSCPNs were subsequently used to prepare a WSCPNs@MnO probe via in situ synthesis, resulting in efficient fluorescence resonance energy transfer between WSCPNs and MnO₂.

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