The gold standard protocol for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection detection remains reverse transcription quantitative polymerase chain reaction (qRT-PCR), which detects viral RNA more sensitively than any other approach. Here, we present Homebrew, a low-cost protocol to extract RNA using widely available reagents. Homebrew is as sensitive as commercially available RNA extraction kits. Homebrew allows for sample pooling and can be adapted for automation in high-throughput settings. For complete details on the use and execution of this protocol, please refer to Page et al. (2022).
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8938258 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2022.101300 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Development of a portable multi-step microfluidic device for point-of-care nucleic acid diagnostics.
Anal Chim Acta
January 2025
State Key Laboratory of Microbial Technology, Microbial Technology Institute, Shandong University, Qingdao, Shandong, 266237, China. Electronic address:
Background: The COVID-19 pandemic has significantly affected global health, economies, and societies, and highlighted the urgent need for rapid, sensitive, affordable, and portable diagnostic devices for respiratory diseases, especially in areas with limited resources. In recent years, there has been rapid development in integrated equipments using microfluidic chips and biochemical detection technologies. However, these devices are expensive and complex to operate, showing limited feasibility for in point of care tests (PoCTs).
View Article and Find Full Text PDFThe legal framework for the production of alcohol for personal use within the European Union.
Nordisk Alkohol Nark
August 2024
Technische Universität Dresden, Dresden, Germany; Institute for Mental Health Policy Research, Centre for Addiction and Mental Health, Toronto, Canada; Campbell Family Mental Health Research Institute, Centre for Addiction and Mental Health, Toronto, Canada; Department of Psychiatry, University of Toronto, Toronto, Canada; Dalla Lana School of Public Health & Department of Psychiatry, University of Toronto, Toronto, Canada; Center for Interdisciplinary Addiction Research, Department of Psychiatry and Psychotherapy, University Medical Center Hamburg-Eppendorf, Hamburg, Germany; and Programm on Substance Abuse & WHO Collaborating Centre, Public Health Agency of Catalonia, Barcelona, Spain.
Article Synopsis
- The paper analyzes the legal rules surrounding the production of alcohol for personal use in EU Member States, highlighting that 12 countries allow tax-free production.
- It mentions that some countries limit the quantity, primarily focusing on beer and wine, with only one country exempting spirits from tax.
- The study reveals that legal exemptions exist in about half of the EU, raising concerns about unreported alcohol consumption and the need to balance economic benefits with public health risks.
Early Diagnosis of Colorectal Cancer Based on Bisulfite-free Site-specific Methylation Identification PCR Strategy: High-Sensitivity, Accuracy, and Primary Medical Accessibility.
Adv Sci (Weinh)
September 2024
School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, P. R. China.
Due to its decade-long progression, colorectal cancer (CRC) is most suitable for population screening to achieve a significant reduction in its incidence and mortality. DNA methylation has emerged as a potential marker for the early detection of CRC. However, the current mainstream methylation detection method represented by bisulfite conversion has issues such as tedious operation, DNA damage, and unsatisfactory sensitivity.
View Article and Find Full Text PDFEnvironmental DNA Isolation, Validation, and Preservation Methods.
Methods Mol Biol
April 2024
Division of Invertebrate Zoology, American Museum of Natural History, New York, NY, USA.
Environmental DNA (eDNA) workflows contain many familiar molecular-lab techniques, but also employ several unique methodologies. When working with eDNA, it is essential to avoid contamination from the point of collection through preservation and select a meaningful negative control. As eDNA can be obtained from a variety of samples and habitats (e.
View Article and Find Full Text PDFHomebrew Photolithography for the Rapid and Low-Cost, "Do It Yourself" Prototyping of Microfluidic Devices.
ACS Omega
September 2023
Interdisciplinary Computing and Complex BioSystems, ICOS, Newcastle University, Newcastle upon Tyne NE4 5TG, U.K.
Photolithography is the foundational process at the root of micro-electromechanical (MEMS) and microfluidic systems manufacture. The process is descendant from the semiconductor industry, originating from printed circuit board and microprocessor fabrication, itself historically performed in a cleanroom environment utilizing expensive, specialist microfabrication equipment. Consequently, these conditions prove cost-prohibitive and pose a large barrier to entry.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!