AI Article Synopsis
- The study investigates how various environmental factors, such as temperature and nutrition, influence the different larval stages of Caenorhabditis elegans postembryonic development.
- Results indicate that each larval stage reacts uniquely to these factors, suggesting that developmental timing is finely tuned by stage-specific events.
- Understanding these mechanisms may enhance our knowledge of how developmental timing is regulated in organisms.
Article Abstract
Background: After embryonic development, Caenorhabditis elegans progress through for larval stages, each of them finishing with molting. The repetitive nature of C. elegans postembryonic development is considered an oscillatory process, a concept that has gained traction from regulation by a circadian clock gene homologue. Nevertheless, each larval stage has a defined duration and entails specific events. Since the overall duration of development is controlled by numerous factors, we have asked whether different rate-limiting interventions impact all stages equally.
Results: We have measured the duration of each stage of development for over 2500 larvae, under varied environmental conditions known to alter overall developmental rate. We applied changes in temperature and in the quantity and quality of nutrition and analysed the effect of genetically reduced insulin signalling. Our results show that the distinct developmental stages respond differently to these perturbations. The changes in the duration of specific larval stages seem to depend on stage-specific events. Furthermore, our high-resolution measurement of the effect of temperature on the stage-specific duration of development has unveiled novel features of temperature dependence in C. elegans postembryonic development.
Conclusions: Altogether, our results show that multiple factors fine tune developmental timing, impacting larval stages independently. Further understanding of the regulation of this process will allow modelling the mechanisms that control developmental timing.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9047341 | PMC |
| http://dx.doi.org/10.1186/s12915-022-01295-2 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Experience-dependent, sexually dimorphic synaptic connectivity defined by sex-specific cadherin expression.
Sci Adv
November 2024
Department of Biological Sciences, Columbia University, Howard Hughes Medical Institute, New York, NY 10027, USA.
Early-life experience influences subsequent maturation and function of the adult brain, sometimes even in a sex-specific manner, but underlying molecular mechanisms are poorly understood. We describe here how juvenile experience defines sexually dimorphic synaptic connectivity in the adult nervous system. Starvation of juvenile males disrupts serotonin-dependent activation of the CREB transcription factor in a nociceptive sensory neuron, PHB.
View Article and Find Full Text PDFImpact of photobleaching on quantitative, spatio-temporal, super-resolution imaging of mitochondria in live larvae.
Npj Imaging
November 2024
Research Department of Cell and Developmental Biology, Division of Biosciences, The Centre for Cell and Molecular Dynamics, University College London, London, UK.
Super-resolution (SR) 3D rendering allows superior quantitative analysis of intracellular structures but has largely been limited to fixed or ex vivo samples. Here we developed a method to perform SR live imaging of mitochondria during post-embryonic development of . larvae.
View Article and Find Full Text PDFFree long-chain fatty acids trigger early postembryonic development in starved Caenorhabditis elegans by suppressing mTORC1.
PLoS Biol
October 2024
School of Life Science and Technology, ShanghaiTech University, Shanghai, China.
Postembryonic development of animals has long been considered an internally predetermined program, while macronutrients were believed to be essential solely for providing biomatters and energy to support this process. However, in this study, by using a nematode Caenorhabditis elegans (abbreviated as C. elegans hereafter) model, we surprisingly discovered that dietary supplementation of palmitic acid alone, rather than other abundant essential nutrients such as glucose or amino acid mixture, was sufficient to initiate early postembryonic development even under complete macronutrient deprivation.
View Article and Find Full Text PDFNeurogenesis in Caenorhabditis elegans.
Genetics
October 2024
Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Animals rely on their nervous systems to process sensory inputs, integrate these with internal signals, and produce behavioral outputs. This is enabled by the highly specialized morphologies and functions of neurons. Neuronal cells share multiple structural and physiological features, but they also come in a large diversity of types or classes that give the nervous system its broad range of functions and plasticity.
View Article and Find Full Text PDFA fluorescent reporter for rapid assessment of autophagic flux reveals unique autophagy signatures during post-embryonic development and identifies compounds that modulate autophagy.
Autophagy Rep
July 2024
Department of Pediatrics, Washington University in St Louis School of Medicine, St Louis, MO 63110, USA.
Autophagy is important for many physiological processes; and disordered autophagy can contribute to the pathogenesis of a broad range of systemic disorders. is a useful model organism for studying the genetics of autophagy, however, current methods for studying autophagy are labor-intensive and not readily amenable to high-throughput procedures. Here we describe a fluorescent reporter, GFP::LGG-1::mKate2, which is useful for monitoring autophagic flux in live animals.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!