AI Article Synopsis
- Recent advancements in tissue clearing technologies allow researchers to examine the entire mouse brain in detail, creating a need for effective data analysis tools.
- CUBIC-Cloud is introduced as a cloud-based system that automates the analysis, visualization, and integration of whole-brain datasets for users.
- The platform has been applied in various studies, including analyzing cell type distribution, Alzheimer's disease plaque deposition, neuronal activity during inflammation, and mapping brain connectivity.
Article Abstract
Recent advancements in tissue clearing technologies have offered unparalleled opportunities for researchers to explore the whole mouse brain at cellular resolution. With the expansion of this experimental technique, however, a scalable and easy-to-use computational tool is in demand to effectively analyze and integrate whole-brain mapping datasets. To that end, here we present CUBIC-Cloud, a cloud-based framework to quantify, visualize, and integrate mouse brain data. CUBIC-Cloud is a fully automated system where users can upload their whole-brain data, run analyses, and publish the results. We demonstrate the generality of CUBIC-Cloud by a variety of applications. First, we investigated the brain-wide distribution of five cell types. Second, we quantified Aβ plaque deposition in Alzheimer's disease model mouse brains. Third, we reconstructed a neuronal activity profile under LPS-induced inflammation by c-Fos immunostaining. Last, we show brain-wide connectivity mapping by pseudotyped rabies virus. Together, CUBIC-Cloud provides an integrative platform to advance scalable and collaborative whole-brain mapping.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9017177 | PMC |
| http://dx.doi.org/10.1016/j.crmeth.2021.100038 | DOI Listing |
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