AI Article Synopsis
- Persistence of HIV-1 latent reservoir cells during antiretroviral therapy (ART) presents a significant challenge in curing HIV-1.
- * Researchers developed the "widely distributed intact provirus elimination" (WIPE) assay, a long-term cell culture system that mimics the diverse infection scenarios of HIV-1.
- * The WIPE assay allows for the evaluation of latency-reversing agents (LRAs) and demonstrates that combining LRAs with ART can effectively reactivate and help eliminate latent HIV-1 cells.
Article Abstract
Persistence of HIV-1 latent reservoir cells during antiretroviral therapy (ART) is a major obstacle for curing HIV-1. Even though latency-reversing agents (LRAs) are under development to reactivate and eradicate latently infected cells, there are few useful models for evaluating LRA activity . Here, we establish a long-term cell culture system called the "widely distributed intact provirus elimination" (WIPE) assay. It harbors thousands of different HIV-1-infected cell clones with a wide distribution of HIV-1 provirus similar to that observed . Mathematical modeling and experimental results from this infection model demonstrates that the addition of an LRA to ART shows a latency-reversing effect and contributes to the eradication of replication-competent HIV-1. The WIPE assay can be used to optimize therapeutics against HIV-1 latency and investigate mechanistic insights into the clonal selection of heterogeneous HIV-1-infected cells.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9017183 | PMC |
| http://dx.doi.org/10.1016/j.crmeth.2021.100122 | DOI Listing |
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