AI Article Synopsis
- * The authors developed four new mouse lines that use CreER-recombination to tag neuron subsets based on their differentiation timing, allowing for active experimentation on these neurons in live animals.
- * They created a publicly accessible brain atlas (NeuroGT) that contains images of the tagged neurons and their features throughout the brain, helping researchers find relevant tagging information for their studies.
Article Abstract
Neuronal birthdate is one of the major determinants of neuronal phenotypes. However, most birthdating methods are retrospective in nature, allowing very little experimental access to the classified neuronal subsets. Here, we introduce four neurogenic tagging mouse lines, which can assign CreER- recombination to neuron subsets that share the same differentiation timing in living animals and enable various experimental manipulations of the classified subsets. We constructed a brain atlas of the neurogenic tagging mouse lines (NeuroGT), which includes holistic image data of the -recombined neurons and their processes across the entire brain that were tagged on each single day during the neurodevelopmental period. This image database, which is open to the public, offers investigators the opportunity to find specific neurogenic tagging driver lines and the stages of tagging appropriate for their own research purposes.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9017123 | PMC |
| http://dx.doi.org/10.1016/j.crmeth.2021.100012 | DOI Listing |
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Article Synopsis
- * The authors developed four new mouse lines that use CreER-recombination to tag neuron subsets based on their differentiation timing, allowing for active experimentation on these neurons in live animals.
- * They created a publicly accessible brain atlas (NeuroGT) that contains images of the tagged neurons and their features throughout the brain, helping researchers find relevant tagging information for their studies.
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