In this work, the ammonium formate version of the QuEChERS method has been applied for the first time to the extraction of a group of nine phthalic acid esters and one adipate from three types of coffee (maximum intensity, intermediate intensity and decaffeinated) prepared from coffee capsules, using gas chromatography coupled to mass spectrometry for analytes separation and determination. Matrix-matched calibration showed good linearity with determination coefficients (R) higher than 0.9983 for all analytes and matrices. In general, matrix effect assessment revealed a medium effect of signal suppression, while mean relative recovery values were in the range 70-120% with relative standard deviation values ≤19% for most analytes. Several samples of each type of coffee obtained from capsules made of different materials were also analysed, finding concentrations of DBP, DEHA and DEHP in the range 29.3-734 ng/capsule, below the tolerable daily intake established for some of them.
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Article Synopsis
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- Fifteen male players tested caffeinated coffee, decaffeinated coffee (placebo), powdered caffeine, and a powdered placebo in a controlled crossover study, followed by a Wingate test and blood sample analysis.
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