AI Article Synopsis
- Inflammatory processes are crucial in the early stages of diabetic nephropathy (DN) development.
- Researchers use adult kidney (AK) explants from Type 1 diabetic animal models to study the disease's mechanisms while maintaining the kidney's structural integrity.
- The study highlights how AK explants can effectively mimic DN progression and allow for testing potential treatments that target inflammation related to the disease.
Article Abstract
Inflammatory processes play a central role in the pathogenesis of diabetic nephropathy (DN) in the early stages of the disease. In vitro approach using cell lines help to understand the mechanisms involves and allow the molecular and biochemical processes. Adult kidney (AK) explants remain an essential instrument for advancing our understanding of the molecular and cellular regulation of signalling pathways from an organotipic view with physiological system interaction integrated. AK explants from T1DM animal model (BB rat) are obtained by slicing central kidney area preserving the organ's cytoarchitecture and reproduce the classical events detected during the DN in an in vivo model such as inflammation, epithelial-mesenchymal transition (EMT) processes by the modulation of a-SMA and e-Cadherin among others which have been determined by qRT-PCR, western-blot and immunohistochemistry. In this regard, AK explants reproduce the signalling pathways involve in DN progression (proinflammatory NFkB and inflammasome complex). This work demonstrates AK explants is a physiological experimental approach for studying the development and progression of DN. Furthermore, the inflammatory processes in AK explants under a diabetic environment and/or BB rats could be modulated by potential treatments for DN.
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| http://dx.doi.org/10.1016/j.lfs.2022.120575 | DOI Listing |
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