Complex carbohydrates shape the gut microbiota, and the collective fermentation of resistant starch by gut microbes positively affects human health through enhanced butyrate production. The keystone species () is a specialist in degrading resistant starch; its degradation products are used by other bacteria including (). We analysed the metabolic and spatial relationships between and during potato starch degradation and found that utilizes glucose that is released from upon degradation of resistant potato starch and soluble potato amylopectin. Additionally, we found that produces a halo of glucose around it when grown on solid media containing potato amylopectin and that cells deficient for growth on potato amylopectin (∆) can grow within the halo. Furthermore, when these ∆ cells grow within this glucose halo, they have an elongated cell morphology. This long-cell phenotype depends on the glucose concentration in the solid media: longer cells are formed at higher glucose concentrations. Together, our results indicate that starch degradation by cross-feeds other bacteria in the surrounding region by releasing glucose. Our results also elucidate the adaptive morphology of cells under different nutrient and physiological conditions.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1099/mic.0.001180 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Association and substitution analyses of dietary sugars, starch and fiber for indices of body fat and cardiometabolic risk- a NoHoW sub-study.
Eur J Nutr
January 2025
Department of Public Health, Section for General Practice, University of Copenhagen, Copenhagen, Denmark.
Purpose: To examine the associations and substitutions of dietary sugars [extrinsic (free) or intrinsic (non-free)] as well as dietary starch and fiber intakes for indices of body fat and cardiometabolic health.
Methods: Dietary intake was assessed at multiple times using multi-day 24-hour recalls over 18-months for indices of body fat (body fat %, waist circumference, BMI, and weight change) (n = 1066) and at baseline and 12 months for cardiometabolic outcomes (LDL, HDL, HbA1c) (n = 736). Bayesian modeling was applied to analyze the probabilistic impact of dietary carbohydrate components using credible intervals for association and substitution analyses with repeated measures random effects modeling.
Phenotypic and Complete Reference Whole Genome Sequence Analyses of Two spp. Isolates from a Gray Wolf () Gastrointestinal Tract.
Vet Sci
January 2025
Biology Program, Oregon State University-Cascades, 1500 SW Chandler Avenue, Bend, OR 97702, USA.
Inflammatory bowel disease (IBD) is increasing among mammals around the world, and domestic dogs are no exception. There is no approved cure for canine IBD with limited treatment options. Novel probiotic bacteria discovery from free-ranging animals for the treatment of IBD in domestic pets can likely yield promising probiotic candidates.
View Article and Find Full Text PDFTranscriptome Analysis Revealed the Molecular Mechanism of Acetic Acid Increasing Pigment Production in CICC41233.
J Fungi (Basel)
January 2025
School of Life Sciences, Jiangxi Science and Technology Normal University, Nanchang 330013, China.
The addition of acetic acid to cultures is usually used to inhibit the growth of heterotrophic bacteria; however, we found that acetic acid also promotes the growth of CICC41233, as well as the synthesis of pigments (MPs). Compared with no acetic acid or HCl addition, the diameter of CICC41233 colonies increased significantly under acetic acid conditions. On the sixth day of fermentation, the yield of total pigments in increased significantly by 9.
View Article and Find Full Text PDFSaprotrophic Wood Decay Ability and Plant Cell Wall Degrading Enzyme System of the White Rot Fungus : Secretome, Metabolome and Genome Investigations.
J Fungi (Basel)
December 2024
A.N. Bach Institute of Biochemistry, Research Center of Biotechnology, Russian Academy of Sciences, Moscow 119071, Russia.
The basidiomycete strain LE-BIN1700 (Agaricales, ) is able to grow on agar media supplemented with individual components of lignocellulose such as lignin, cellulose, xylan, xyloglucan, arabinoxylan, starch and pectin, and also to effectively destroy and digest birch, alder and pine sawdust. produces a unique repertoire of proteins for the saccharification of the plant biomass, including predominantly oxidative enzymes such as laccases (family AA1_1 CAZymes), GMC oxidoreductases (family AA3_2 CAZymes), FAD-oligosaccharide oxidase (family AA7 CAZymes) and lytic polysaccharide monooxygenases (family LPMO X325), as well as accompanying acetyl esterases and loosenine-like expansins. Metabolomic analysis revealed that, specifically, monosaccharides and carboxylic acids were the key low molecular metabolites in the culture liquids in the experimental conditions.
View Article and Find Full Text PDFChemical Diversity of UK-Grown Tea Explored Using Metabolomics and Machine Learning.
Metabolites
January 2025
Department of Life Sciences, Aberystwyth University, Aberystwyth SY23 3DA, UK.
Background/objectives: Dartmoor Estate Tea plantation in Devon, UK, is renowned for its unique microclimate and varied soil conditions, which contribute to the distinctive flavours and chemical profiles of tea. The chemical diversity of fresh leaf samples from various garden locations was explored within the plantation.
Methods: Fresh leaf, which differed by location, cultivar, time of day, and variety, was analysed using Flow Infusion Electrospray Ionisation Mass Spectrometry (FIE-MS).
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!