sp. nov and an anti-ginger plague agent sp. nov, two novel species isolated from soil of .

Two novel strains of actinobacteria, ZYC-3 and BH-SS-21, were isolated from Hunan Province, PR China. The fermentation broth of BH-SS-21 inhibited the rapid spread of ginger blast, unlike that of ZYC-3. The taxonomic characteristics of ZYC-3 and BH-SS-21 were defined using a polyphasic approach. The analysis of the full-length 16S rRNA gene sequence revealed that ZYC-3 and BH-SS-21 represented members of the genus . ZYC-3 had less than 98.7% sequence similarities to all species of the genus , while BH-SS-21 exhibited 99.97, 98.95, 98.83, 98.82, 98.75 and less than 98.7% sequence similarities to '' A217, JCM 4958, NBRC 15418, NBRC 13017, JCM 4521 and other species with validly published names in the genus . However, the digital DNA-DNA relatedness and average nucleotide identity values between ZYC-3, BH-SS-21, and their closely related strains were significantly lower than the recommended threshold values. Also, phenotypic, chemotaxonomic and genetic features distinguished ZYC-3 and BH-SS-21 from their reference strains. On the basis of their genotypic and phenotypic characteristics, strains ZYC-3 and BH-SS-21 were classified as representing novel species of the genus under the names sp. nov. ZYC-3 (=CICC 25040=JCM 34560=MCCC 1K04978) and sp. nov. BH-SS-21 (=MCCC 1K06236=JCM 34767), respectively.