Anticandidal efficacy on polymide based denture resin using Photodynamic therapy, chemical and herbal disinfectants and their effect on surface roughness and hardness.

Aim: The present study aimed to evaluate the anticandidal effectiveness of PDT, DL, Triphala, CHX, and NE and their effects on Ra and the hardness of polyamide denture base colonized with C. Albicans.

Material And Methods: A total of 50 thermo-injected polyamide denture resins were constructed and inoculated by the American Type Culture Collection (ATCC) of C. albicans in an in-vitro setup. The specimens were arbitrarily allocated into five groups, pre-candida count was measured then subjected to the diverse polyamide denture disinfection methodologies: Group 1: PDT, Group 2: Diode Laser, group 3: Triphala, group 4: 0.12% CHX (Control) and group 5 Neem extract. After disinfection protocol, post-candida count (CFU/ml) was assessed. Surface roughness and surface hardness of polyamide dentures were evaluated and statistical differences in the Ra and Vickers hardness was also assessed. Statistical analysis was performed for CFU/mL (log10) for exposed C. albicans by two-way ANOVA and Tukey's multiple test (p>0.05). For normality of the data, Kolmogorov Smirnov test was executed.

Results: The highest anti-microbial efficacy against Candida colonies was displayed by chemical control group 0.12% CHX (11.39 ± 1.8 CFU/ml). This was comparable to herbal NE (12.45 ± 2.9 CFU/ml) (p>0.05). There was no statistical difference found in the surface hardness values among the disinfected groups. Group 2: DL (1.32 ± 0.13 μm) showed the highest Ra value comparable to group 1: PDT (1.21 ± 0.22 μm) CONCLUSION: Polyamide denture base colonized with C. Albicans and disinfected with 0.12% Chlorhexidine and Neem extract demonstrated the highest antimicrobial efficacy with decreased surface roughness and no alteration in denture hardness.