Cultivation location, maturity levels, and extraction solvents could affect the bioactive compounds and biological activities of mulberry ( Linnaeus). The lack of study on Malaysia-grown mulberry causes its underutilization. This study investigated the bioactive compound content and the antioxidant activity of Sabah-grown mulberry at two different maturity stages (fruits: red mature and black fully ripe; leaves: young and mature) extracted using 70% (/) methanol, 60% (/) ethanol, and 65% (/) acetone. Analyses showed that mulberry fruits demonstrated maturity-dependent increment (except UHPLC-DAD quantification), while the leaves revealed maturity-dependent reduction. Principal component analysis (PCA) displayed 65% (/) acetone black fully ripe fruits as the best phenolics and antioxidant sources. However, the 60% (/) ethanol black fully ripe fruits contained 20.08-68.43% higher total anthocyanins. Meanwhile, the 65% (/) acetone and 70% (/) methanol red mature fruits were higher in chlorogenic acid (27.53-47.12%) and rutin (31.42-35.92%) than other fruit extracts, respectively. For leaves, 65% (/) acetone young leaves were the best phenolics and antioxidant sources. However, the 60% (/) ethanol young leaves possessed greater chlorogenic acid (19.56-74.11%) than other leaf extracts. Overall, Malaysia-grown mulberry is rich in phenolics and antioxidants, suggesting its potential application in food and pharmaceutical products.
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http://dx.doi.org/10.3390/molecules27082406 | DOI Listing |
Bioresour Technol
January 2025
Department of Chemical Engineering (BK21 FOUR Integrated Engineering), Kyung Hee University, Yongin-si, Gyeonggi-do 17104, Republic of Korea. Electronic address:
Polyhydroxybutyrate (PHB) production using methanotrophs offers an economical solution to counter increasing environmental pollution. However, the substrate specificity of methanotrophs limits their ability to use multiple gases for chemical production. In this study, a synthetic heterotrophic and methanotrophic co-culture system was developed to co-utilize methane and propane for PHB production.
View Article and Find Full Text PDFJ Mol Model
January 2025
Laboratory of Nanostructures and Advanced Materials, Mechanics and Thermofluids, Faculty of Sciences and Technologies, Hassan II University of Casablanca, B.P 146, 20650, Mohammedia, Morocco.
Context: Designing efficient sensitive materials for the detection of volatile organic compounds (VOCs) such as ethanol, acetone, and benzene is stringent owing to the significant environmental and health risks induced by these compounds, in addition to their role as biomarkers for chronic diseases and food quality. This study investigates the adsorption mechanisms of VOC molecules (ethanol, acetone, and benzene) on both non-oxidized and oxidized SnO (110) monolayers and identifies the most suitable surface for gas sensing applications. For this, we examined structural properties, adsorption energies, density of states, gas responses, and recovery times.
View Article and Find Full Text PDFFood Chem X
January 2025
Department of Food Science, Rutgers University, 65 Dudley Road, New Brunswick, NJ 08901, USA.
During the extraction of membrane proteins from oil bodies (OBs), organic solvents dissolve the lipid core and precipitate proteins through solvent stress. Here the effects of solvent type and defatting sequence on the composition and structure of membrane proteins were investigated SDS-PAGE, FTIR, and SEM-EDS. High purity oleosin (86 %) was obtained by treatment first with a Floch solution and then with cold acetone and petroleum ether after twice washing OBs with urea.
View Article and Find Full Text PDFBioresour Technol
January 2025
College of Bioscience and Bioengineering, Institute of Applied Microbiology, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China. Electronic address:
Int J Nanomedicine
December 2024
Tecnologico de Monterrey, Institute for Obesity Research, Monterrey, México.
Introduction: Disulfiram (DSF) reduces insulin resistance and weight gain in obese mice. However, the effect on adipose tissue is unexplored due to their high instability under physiological conditions, limiting clinical applications. Thus, it is meaningful to develop a DSF carrier for sustained release to adipose tissue.
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