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We assessed the viability of self-sampled gargle water direct RT-LAMP (LAMP) for detecting SARS-CoV-2 infections by estimating its sensitivity with respect to the gold standard indirect RT-PCR of paired oro-nasopharyngeal swab samples. We also assessed the impact of symptom onset to test time (STT)-i.e., symptom days at sampling, on LAMP. In addition, we appraised the viability of gargle water self-sampling versus oro-nasopharyngeal swab sampling, by comparing paired indirect RT-PCR results. 202 oro-nasopharyngeal swab and paired self-sampled gargle water samples were collected from hospital patients with COVID-19 associated symptoms. LAMP, indirect and direct RT-PCR were performed on all gargle water samples, and indirect RT-PCR was performed on all oro-nasopharyngeal samples. LAMP presented a sensitivity of 80.8% (95% CI: 70.8-90.8%) for sample pairs with sub-25 Ct oro-nasopharyngeal indirect RT-PCR results, and 77.6% (66.2-89.1%) sensitivity for sub-30 Ct samples with STT ≤ 7 days. STT, independently of Ct value, correlated negatively with LAMP performance. 80.7% agreement was observed between gargle water and oro-nasopharyngeal indirect RT-PCR results. In conclusion, LAMP presents an acceptable sensitivity for low Ct and low STT samples. Gargle water may be considered as a viable sampling method, and LAMP as a screening method, especially for symptomatic persons with low STT values.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9030430 | PMC |
| http://dx.doi.org/10.3390/diagnostics12040775 | DOI Listing |
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Article Synopsis
- The study investigated the effects of cetylpyridinium chloride (CPC) and on-demand aqueous chlorine dioxide mouthwash on the SARS-CoV-2 viral load in mildly symptomatic COVID-19 patients.
- In a randomized clinical trial, participants were assigned to one of three groups: CPC mouthwash, chlorine dioxide mouthwash, or a placebo.
- Results showed that CPC mouthwash significantly reduced salivary viral titer at 10 minutes but had no effect at later intervals, whereas chlorine dioxide mouthwash showed no significant difference compared to the placebo at any time point.
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