AI Article Synopsis
- The study compared two lines of plants (HOCR with high oleic acid and LOCR with low oleic acid) to analyze lysine acetylation and its effects on fatty acid metabolism.
- The research identified 45 proteins with different levels of acetylation, mainly linked to processes like photosynthesis and carbon fixation.
- Key genes related to fatty acid metabolism were pinpointed, and this study is the first to show that acetylation plays a role in regulating oleic acid levels in rapeseed.
Article Abstract
In this study, lysine acetylation analysis was conducted using two near-isogenic lines, HOCR and LOCR, containing high and low oleic acid contents, respectively, to explore this relationship. Proteins showing differences in quantitative information between the lines were identified in lysine acetylation analysis, and KEGG pathways were analyzed, yielding 45 enriched proteins, most of which are involved in carbon fixation in photosynthetic organisms, photosynthesis, ascorbate and aldarate metabolism, and glycolysis. Potential key genes related to fatty acid metabolisms were determined. To further explore the effect of acetylation modification on fatty acid metabolisms, the acyl-ACP3 related gene was cloned, and a base mutation at No.63 was changed via overlapping primer PCR method. This study is the first to demonstrate that acetylation modification can regulate oleic acid metabolisms, which provides a promising approach for the study of the molecular mechanism of oleic acid in rapeseed.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9029296 | PMC |
| http://dx.doi.org/10.3390/biology11040483 | DOI Listing |
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