A PHP Error was encountered

Severity: Warning

Message: fopen(/var/lib/php/sessions/ci_sessioneg2jr57f0r59f20qjcbj2bmqjhpgav4l): Failed to open stream: No space left on device

Filename: drivers/Session_files_driver.php

Line Number: 177

Backtrace:

File: /var/www/html/index.php
Line: 316
Function: require_once

A PHP Error was encountered

Severity: Warning

Message: session_start(): Failed to read session data: user (path: /var/lib/php/sessions)

Filename: Session/Session.php

Line Number: 137

Backtrace:

File: /var/www/html/index.php
Line: 316
Function: require_once

A PHP Error was encountered

Severity: Warning

Message: file_get_contents(https://...@gmail.com&api_key=61f08fa0b96a73de8c900d749fcb997acc09): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests

Filename: helpers/my_audit_helper.php

Line Number: 143

Backtrace:

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 143
Function: file_get_contents

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 209
Function: simplexml_load_file_from_url

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3098
Function: getPubMedXML

File: /var/www/html/application/controllers/Detail.php
Line: 574
Function: pubMedSearch_Global

File: /var/www/html/application/controllers/Detail.php
Line: 488
Function: pubMedGetRelatedKeyword

File: /var/www/html/index.php
Line: 316
Function: require_once

A PHP Error was encountered

Severity: Warning

Message: Attempt to read property "Count" on bool

Filename: helpers/my_audit_helper.php

Line Number: 3100

Backtrace:

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3100
Function: _error_handler

File: /var/www/html/application/controllers/Detail.php
Line: 574
Function: pubMedSearch_Global

File: /var/www/html/application/controllers/Detail.php
Line: 488
Function: pubMedGetRelatedKeyword

File: /var/www/html/index.php
Line: 316
Function: require_once

First Report of Causing Collar Rot of Guar () in Mexico. | LitMetric

First Report of Causing Collar Rot of Guar () in Mexico.

Plant Dis

Centro de Investigación en Alimentación y Desarrollo, Coordinación Culiacán, Carretera El Dorado Km 5.5, Campo el Diez, Culiacán, Sinaloa, Mexico, 80110;

Published: April 2022

AI Article Synopsis

  • - Observations of collar rot in guar fields in Sinaloa, Mexico revealed symptoms like wilting and reduced growth, with disease incidence between 15% to 40% and a notable presence of white mycelium and brown sclerotia at the plants’ bases.
  • - Fungal isolation from diseased plants involved surface sterilization and culturing on PDA medium, resulting in white, cottony colonies with dark brown sclerotia, which were characterized microscopically and purified using the hyphal-tip method.
  • - Molecular analysis including DNA extraction and sequencing of the ITS region confirmed the identity of the fungus with 99.21% to 100% similarity to known sequences, and the isolate was deposited in a culture

Article Abstract

(Fabaceae), also known as guar or cluster bean, is an annual legume grown mainly for industrial purposes and also as an ingredient for animal feed. In October 2021, collar rot symptoms were observed in five guar fields located in Guasave, Sinaloa, Mexico. Abundant white mycelium, and later brown and small sclerotia were observed at the base of the stems. Diseased plants showed reduced growth, wilting, and drying of the entire plant. Disease incidence ranged from 15 to 40%. Samples were collected from each field at two phenological stages (vegetative and reproductive). For fungal isolation, symptomatic stems pieces were surface sterilized with 2% sodium hypochlorite for 2 min, rinsed in sterilized distilled water two times, placed on PDA medium and incubated at 28°C in darkness for 3 days. Sclerotium-like colonies were consistently obtained and five isolates from five different fields were purified by the hyphal-tip method. Fungal colonies were white, cottony, and often forming fans. Sclerotia (1 to 2 mm diameter) were white at first and then gradually turned dark brown. Microscopic examination showed septate hyphae with some cells having clamp connections. A representative isolate was deposited in the Culture Collection of Phytopathogenic Fungi of the Faculty of Agriculture of Fuerte Valley at the Sinaloa Autonomous University under Accession no. FAVF647. For molecular identification, genomic DNA was extracted, and the internal transcribed spacer (ITS) region was amplified by PCR and sequenced using the primer pair ITS5/ITS4 (White et al. 1990). The sequence was deposited in GenBank (accession no. OM510466). BLASTn searches in GenBank showed 99.21 to 100% identity with the available sequences of (accession nos. MK926446, MH854711, and KY175225). A phylogenetic analysis using the maximum Likelihood method placed isolate FAVF647 in the same clade as . Pathogenicity tests were performed by inoculating 10 healthy guar seedlings (15-day-old) grown in pots. Four sclerotia were directly placed on the stem base of each plant. Five uninoculated guar seedlings were used as control. All plants were placed in a moist chamber at 25°C with a 12-h photoperiod for 2 days. Collar rot symptoms appeared on inoculated plants after 3 days, whereas control plants remained symptomless. Pathogenicity test was performed twice with similar results. The fungus was reisolated from the artificially inoculated plants, thus fulfilling Koch's postulates. has been reported on guar plants in Australia, Brazil, Fiji, India, and the United States (Farr and Rossman 2022). To our knowledge, this is the first report of causing collar rot of guar in Mexico. The disease is very common in guar fields in Sinaloa, Mexico, therefore additional studies are needed to develop effective disease-management strategies.

Download full-text PDF

Source
http://dx.doi.org/10.1094/PDIS-02-22-0270-PDNDOI Listing

Publication Analysis

Top Keywords

collar rot
16
report causing
8
causing collar
8
guar
8
rot guar
8
guar mexico
8
rot symptoms
8
guar fields
8
sinaloa mexico
8
guar seedlings
8

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!