gen. nov., sp. nov., isolated from larvae of mealworm L., and a proposal to transfer Liu . 2016 to a new genus as comb. nov.

Two enterobacterial strains, designated YMB-R21 and YMB-R22, were isolated from larvae of mealworm L. and examined for their taxonomic characteristics. A 16S rRNA gene-based neighbour-joining tree showed that the two isolates formed two distinct sublineages within the family and were separated from other genera of the family. The isolates showed 16S rRNA gene sequence similarity of 98.9 % to each other and ≤96.5 % to members of the order . The phylogenomic analysis based on 92 singly-copy core genes showed that the two isolates belonged to the family and formed a distinct sublineage at a position located remotely from the genera of the family. The loosely associated members were the type strain of and members of the genus . Average nucleotide identity and digital DNA-DNA hybridization values showed that the isolates represented members of a novel species in the family . The values of amino acid identity between the two isolates and the closest relatives were 74.5-75.0 % with the type strain of and 74.5-74.8 % with the type strains of two species, while showed the amino acid identity values of 76.3-76.5 % with two species. Based on the results obtained here, we propose a new genus with the description of sp. nov. (type strain YMB-R21=KCTC 82597=CCM 9152 and strain YMB-R22=KCTC 82598=CCM 9153), with the transfer of Liu . 2016 to a new genus as comb. nov. (type strain SCU-B244=CGMCC 1.12772=DSM 28222=KCTC 42022).