Development and Evaluation of a Film Forming System Containing Myricetin and Miconazole Nitrate for Preventing Catheter-Related Infection.

catheter-related infection (CRI) is a great challenge in clinic now, mainly due to the difficulty in eradicating the biofilms. In this study, the mechanism of the antibiofilm effect of myricetin (MY) on was illustrated. A film forming system (FFS) containing MY and miconazole nitrate (MN) was developed, optimized, and evaluated. The anti-infection effect of MY+MN@FFS against CRI was investigated . To clarify the mechanism of the action of MY, the influence of MY on each key process of the formation of biofilms was evaluated. To deliver MY and MN into the skin and form a drug reservoir on the surface of the skin, the FFS was used as a carrier and MY+MN@FFS was developed, optimized, and evaluated. After preliminary confirmation of drug safety, a percutaneously inserted CRI mouse model was established to investigate the anti-infection effect of MY+MN@FFS by fluorescence microscopy and scanning electron microscopy on the outer surface of the catheters, hematoxylin/eosin staining, and periodic acid-Schiff staining of the mice skin tissues. MY was found to inhibit the morphological transition of and the secretion of exopolysaccharides, resulting in a reduction in biofilms. MY+MN@FFS exhibited excellent properties and no irritation to mice skin. In an anti-infection study, MY+MN@FFS exhibited an excellent preventive effect against percutaneously inserted CRI. MY+MN@FFS might be a potential approach for effectively preventing percutaneously inserted CRI in clinic.