AI Article Synopsis
- The study focuses on the structure of REP-I, an immunostimulatory polysaccharide from radish leaves, revealing it contains nine different monosaccharides, primarily GalA, Gal, Ara, and Rha.
- REP-I exhibits 13 glycosyl linkages typical of RG-I, suggesting complex structural characteristics.
- The findings suggest that the immunostimulatory properties of REP-I arise from its unique RG-I structure, which includes a distinct main chain and multiple branched side-chains.
Article Abstract
Determining the structure of REPI, an immunostimulatory polysaccharide fraction from radish leaves, is an important health objective. Herein, we show that REP-I contains nine different monosaccharides, including GalA (22.2%), Gal (32.6%), Ara (27.5%), and Rha (10.2%) as main sugars. REP-I was also reacted with β-glucosyl Yariv reagent (29.8%), suggesting the presence of the arabino-β-3,6-galactan. Furthermore, methylated-product analysis revealed that REP-I contains 13 different glycosyl linkages, including 4-linked GalpA (21.0%), 2,4-linked Rhap (7.0%), 4-linked Galp (5.8%), 5-linked Araf (10.1%), and 3,6-linked Galp (7.9%), which are characteristic of RG-I. Microstructural information was obtained by sequential degradation using four linkage-specific glycosylases and β-elimination, with fragments analyzed on the basis of sugar composition, methylation, and MS/MS spectra. The results show that the immunostimulatory activity of REP-I is possibly due to the structure of RG-I, which is composed of a main chain with repeating [→2)-Rhap-(1 → 4)-GalpA-(1→] linkage units and three side-chains: a branched α(1 → 5)arabinan, a β(1 → 4)galactan, and arabino-β-3,6-galactan, which are branched at the C(O)4 position of each Rha residue in the REP-I main chain.
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| http://dx.doi.org/10.1016/j.ijbiomac.2022.04.045 | DOI Listing |
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