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Acute Oxygen-Sensing Mitochondria-Generated Temperature Transients in Rat Carotid Body Type I Cells.
Front Physiol
April 2022
Department of Neuroscience, Cell Biology and Physiology, Wright State University, Dayton, OH, United States.
The Carotid Bodies (CB) are peripheral chemoreceptors that detect changes in arterial oxygenation and, via afferent inputs to the brainstem, correct the pattern of breathing to restore blood gas homeostasis. Herein, preliminary evidence is presented supporting a novel oxygen-sensing hypothesis which suggests CB Type I cell "hypoxic signaling" may in part be mediated by mitochondria-generated thermal transients in TASK-channel-containing microdomains. Distances were measured between antibody-labeled mitochondria and TASK-potassium channels in primary rat CB Type I cells.
View Article and Find Full Text PDFCorrection to: Antibody-labeled gold nanoparticles based immunosensor for the detection of thyroxine hormone.
Anal Sci
June 2022
CSIR-Central Scientific Instruments Organization (CSIR-CSIO), Chandigarh, 160030, India.
Adjacent Cell Marker Lateral Spillover Compensation and Reinforcement for Multiplexed Images.
Front Immunol
September 2021
Department of Pathology, Stanford University, Stanford, CA, United States.
Multiplex imaging technologies are now routinely capable of measuring more than 40 antibody-labeled parameters in single cells. However, lateral spillage of signals in densely packed tissues presents an obstacle to the assignment of high-dimensional spatial features to individual cells for accurate cell-type annotation. We devised a method to correct for lateral spillage of cell surface markers between adjacent cells termed REinforcement Dynamic Spillover EliminAtion (REDSEA).
View Article and Find Full Text PDFOrganization of membrane motor in outer hair cells: an atomic force microscopic study.
Pflugers Arch
February 2010
Biophysics Section, Laboratory of Cellular Biology, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, 5 Research Court, Rockville, MD 20850-3211, USA.
Using atomic force microscopy, we imaged the cytosolic surface of the lateral plasma membrane of outer hair cells from guinea pigs' inner ear. We used a "cell-free" preparation, in which a patch of plasma membrane was firmly attached to a substrate and the cytoplasmic face was exposed. The membrane patches contained densely packed particles whose diameter, after correcting for the geometry of the probing tip, was approximately 10 nm.
View Article and Find Full Text PDF[Establishment of colloidal gold-immunochromatography assay strip for detection of type Asia1 foot-and-mouth disease virus].
Sheng Wu Gong Cheng Xue Bao
May 2009
Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chines Academy of Agricultural Science, Lanzhou 730046, China.
To establish a sensitive, rapid and simple gold immunochromatography assay (GICA) for detecting Asia1 type of foot-and-mouth disease virus (FMDV) from the field samples. The purified anti-FMDV type Asia1 monoclonal antibody labeled with colloidal gold and the goat anti-Guinea pig IgG were wrapped onto nitrocellulose membrane as the test line (T line) and the control line (C line), respectively. The strip was then further optimized.
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