AI Article Synopsis
- - An intermediate called prescopranone was found in the mycelium of Streptomyces avermitilis SUKA, which has a biosynthetic gene cluster for making scopranone but lacks a specific enzyme (sprT).
- - The structure of prescopranone was determined using NMR and other spectral techniques, revealing it has a 2-pyranone ring along with two unusual groups (1-ethyl-1-propenyl and 2-ethylbutyl).
- - Prescopranone was shown to inhibit the activity of alkaline phosphatase when induced by bone morphogenetic protein (BMP) in a mutant cell line that lacks BMP receptors.
Article Abstract
A key intermediate in scopranone biosynthesis, prescopranone, accumulated in the mycelium of Streptomyces avermitilis SUKA carrying the biosynthetic gene cluster for scopranone lacking the sprT encoding the monooxygenase. The structure of prescopranone was elucidated by NMR and other spectral data. Prescopranone consists of a 2-pyranone ring with two atypical scoop-like moieties (1-ethyl-1-propenyl and 2-ethylbutyl groups), which was deduced as a product of the modular polyketide syntheses encoded by sprA, sprB, and sprC. Prescopranone inhibited bone morphogenetic protein (BMP)-induced alkaline phosphatase activity in a BMP receptor mutant cell line.
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| http://dx.doi.org/10.1038/s41429-022-00521-x | DOI Listing |
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