AI Article Synopsis
- Researchers identified a new population of human CD34 hematopoietic stem cells (HSCs) called EPCR HSCs, which exhibit exceptional self-renewal and repopulating abilities, with a frequency of about 1 in 3 cells being stem cells.
- The EPCR HSCs display a unique gene expression profile that allows them to produce multiple blood cell lineages both in living organisms and lab settings.
- This discovery provides a clearer picture of the hierarchy and organization of human hematopoietic stem cells, making it easier to study stem cell behavior in post-natal tissues.
Article Abstract
The heterogeneous nature of human CD34 hematopoietic stem cells (HSCs) has hampered our understanding of the cellular and molecular trajectories that HSCs navigate during lineage commitment. Using various platforms including single cell RNA-sequencing and extensive xenotransplantation, we have uncovered an uncharacterized human CD34 HSC population. These CD34EPCR(CD38/CD45RA) (simply as EPCR) HSCs have a high repopulating and self-renewal abilities, reaching a stem cell frequency of ~1 in 3 cells, the highest described to date. Their unique transcriptomic wiring in which many gene modules associated with differentiated cell lineages confers their multilineage lineage output both in vivo and in vitro. At the single cell level, EPCR HSCs are the most transcriptomically and functionally homogenous human HSC population defined to date and can also be easily identified in post-natal tissues. Therefore, this EPCR population not only offers a high human HSC resolution but also a well-structured human hematopoietic hierarchical organization at the most primitive level.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9018830 | PMC |
| http://dx.doi.org/10.1038/s41467-022-29675-w | DOI Listing |
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