AI Article Synopsis
- Peroxynitrite (ONOO) is a reactive oxygen species important for biological functions and can cause diseases when present in excess.
- A new fluorescent probe called DMX has been developed to detect ONOO levels, showing high sensitivity and selectivity.
- DMX can be used in biological studies to visualize ONOO in living organisms like zebrafish, potentially aiding research on the roles of ONOO in various physiological and pathological processes.
Article Abstract
Peroxynitrite (ONOO) is one of quite critical reactive oxygen species that acts critical roles in a number of diverse biological functions and pathological events. Notably, excessive ONOO will lead to sorts of diseases. Thus, monitoring of endogenous ONOO levels will be conducive to exploring the physiological activities and functions of ONOO. Here, a simple turn-on fluorescent probe named DMX is reported using CN bond as the ONOO recognition site and xanthene as the fluorophore. DMX possessed a good linear dependence with ONOO concentration (0-9 μM), highly sensitive detection (DL = 37 nM), and excellent selectivity towards ONOO. What is more, the biological experiments reveal that DMX is able to be utilized to track exogenous/endogenous ONOO employing confocal laser scanning microscopy. Visualization of ONOO in zebrafish was also successfully conducted, suggesting that DMX might be used to study ONOO roles in vivo. We believe that DMX will have potential for exploring the pivotal role of ONOO during all sorts of physiological and pathological activities.
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| http://dx.doi.org/10.1016/j.saa.2022.121264 | DOI Listing |
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