AI Article Synopsis
- Golden Syrian hamsters are becoming popular for studying the SARS-CoV-2 virus, but there are challenges due to a lack of immunological tools specific to them.
- Researchers developed an ELISA method to specifically detect SARS-CoV-2 antibodies in these hamsters through their immune responses to viral peptides and proteins.
- This new assay allows scientists to measure immune responses more precisely, which could help in creating and testing peptide-based vaccines in hamsters.
Article Abstract
Golden Syrian hamsters are increasingly used as a permissive animal model for SARS-CoV-2 virus studies, but the lack of immunological assays and other immunological reagents for hamsters limits its full potential. Herein, we developed an ELISA method to detect antibodies specific to peptides and proteins derived from SARS-CoV-2 virus in immunized golden Syrian hamsters. Under optimized conditions, this assay quantitates antibodies specific for individual viral peptides, peptide pools, and proteins. Hence, this ELISA method allows investigators to quantitatively assess humoral immune responses at the peptide and protein levels and has potential application in the development of peptide-based vaccines to be tested in hamsters.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9013014 | PMC |
| http://dx.doi.org/10.1016/j.jim.2022.113275 | DOI Listing |
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