AI Article Synopsis
- The study focused on using DNA sequencing to find sex-specific genetic markers in green sunfish (Lepomis cyanellus) to identify their sex through SNPs.
- Despite identifying potential SNPs, the tests on DNA samples showed that these markers did not reliably indicate the fish's phenotypic sex.
- The authors suggest conducting more extensive research on the fish's genome and exploring how environmental factors, like temperature and rearing conditions, influence sex determination.
Article Abstract
We used restriction-site associated DNA sequencing for SNP discovery and genotyping of known-sex green sunfish Lepomis cyanellus DNA samples to search for sex-diagnostic single nucleotide polymorphisms (SNPs) and restriction-site associated sequences present in one sex and absent in the other. The bioinformatic analyses discovered candidate SNPs and sex-specific restriction-site associated sequences that fit patterns of male or female heterogametic sex determination systems. However, when primers were developed and tested, no candidates reliably identified phenotypic sex. The top performing SNP candidate (ZW_218) correlated with phenotypic sex 63.0% of the time and the presence-absence loci universally amplified in both sexes. We recommend further investigations that interrogate a larger fraction of the L. cyanellus genome. Additionally, studies on the effect of temperature and rearing density on sex determination, as well as breeding of sex-reversed individuals, could provide more insights into the sex determination system of L. cyanellus.
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| http://dx.doi.org/10.1111/jfb.15063 | DOI Listing |
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