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Molecular Evolution of Paralogous Cold Shock Proteins in E. coli: A Study of Asymmetric Divergence and Protein Functional Networks.
Mol Biotechnol
January 2025
Amity Institute of Biotechnology, Amity University, Kolkata, India.
Nine homologous Cold Shock Proteins (Csps) have been recognized in the E.coli Cold Shock Domain gene family. These Csps function as RNA chaperones.
View Article and Find Full Text PDFC5aR1-positive adipocytes mediate non-shivering thermogenesis in neonatal mice.
iScience
December 2024
Research Group of Endocrinology & Metabolism, Key Laboratory of Vascular Aging, Ministry of Education, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Brown adipose tissue (BAT) plays an important role in maintaining body temperature in newborn mammals; however, its mechanisms remain poorly understood. Here, we report the identification of a special population of brown adipose tissue-derived stromal cells (ASCs) in neonatal mice that highly express CD45 and can be differentiated into adipocytes with lower thermogenic ability. These CD45 adipocytes also characteristically contained complement C5a receptor 1(C5aR1) on the cell membrane.
View Article and Find Full Text PDFUse of nailfold capillaroscopy for the assessment of patients undergoing digit replantation and revascularization.
Jt Dis Relat Surg
January 2025
İnönü Üniversitesi Tıp Fakültesi Turgut Özal Tıp Merkezi, Ortopedi ve Travmatoloji Anabilim Dalı, 44280 Malatya, Türkiye.
Objectives: In this study, we aimed to evaluate microvascular changes using nailfold capillaroscopy in patients who underwent digit replantation and revascularization.
Patients And Methods: A total of 46 patients (34 males, 12 females; mean age: 45.8±17.
Challenges in the surgical treatment of neuroma in continuity in the upper extremity using human acellular nerve allografts.
J Plast Reconstr Aesthet Surg
November 2024
Department of Hand Surgery, Herlev/Gentofte University Hospital of Copenhagen, Hospitalsvej 1, 2900 Hellerup, Denmark; University of Copenhagen, Faculty of Health and Medical Sciences, Institute of Clinical Medicine, Blegdamsvej 3B, 2200 Copenhagen, Denmark.
The restoration of nerve function after the injury might be complicated by the development of a disorganized fibrous mass-a neuroma. This results in sensory and/or motor deficits and pain that can be severely debilitating. Surgical excision of the painful neuroma may leave a gap, which can be bridged using autografts or allografts.
View Article and Find Full Text PDFCold-active β-galactosidase from Weissella confusa SW1 for the preparation of low-lactose milk.
Int J Food Microbiol
February 2025
College of Life Science, Shandong Normal University, Jinan 250358, China. Electronic address:
β-Galactosidases can be used to degrade lactose in milk to prepare lactose-free milk, which is sweeter than ordinary milk and suitable for people with lactose intolerance. The β-galactosidase gene (WcGal2809) was cloned from Weissella confusa SW1 and successfully expressed in Escherichia coli BL21(DE3). The active WcGal2809 was identified to be a heterodimer composed of two distinct proteins LacL (72.
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