AI Article Synopsis
- A perennial herbal plant, significant for its medicinal value, experienced a severe leaf spot blight outbreak in August 2020, leading to substantial yield losses.
- The researchers utilized Koch's postulates to isolate and identify the pathogen, collecting samples from diseased tissues and analyzing them through biological characteristics and molecular methods.
- Their results confirmed the pathogen as the causative agent of the blight, marking the first documentation of this specific disease affecting the plant in China.
Article Abstract
Background: (Thunb.), a perennial herbal plant in the Araceae family, has great medicinal value and market demand. In August 2020, an outbreak of severe leaf spot blight disease resulted in a huge yield loss of . It is necessary to isolate and identify the pathogens that cause spot blight on .
Methods: In this study, we isolated and identified the pathogens by fulfilling Koch's postulates. Disease samples with typical spot blight symptoms were collected and pathogens were isolated from the diseased tissues. The pathogen was identified based on its biological characteristics and molecular analysis of internal transcribed (rDNA-ITS) and large subunit (LSU) sequences. Phylogenetic tree were constructed using MEGA7 software and pathogenicity tests were performed using inoculation. Finally, the pathogen was recovered and identified from the inoculated plants.
Results: Based on Koch's postulates, we identified the pathogen causing spot blight on as . To our knowledge, this is the first study to explore spot blight on caused by in China.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9013236 | PMC |
| http://dx.doi.org/10.7717/peerj.13278 | DOI Listing |
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