Transporters have been used in the production of plant metabolites in microorganisms. This study introduced a tobacco multidrug and toxic compound extrusion transporter, NtJAT1, into alkaloid-producing Escherichia coli cells. NtJAT1 expression enhanced alkaloid production secretion into the medium by 14 folds. Our findings further demonstrate the usefulness of the transport-engineering approach.
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Transport engineering using tobacco transporter NtJAT1 enhances alkaloid production in Escherichia coli.
Biosci Biotechnol Biochem
June 2022
Laboratory of Medicinal Cell Biology, Kobe Pharmaceutical University, Motoyamakita-machi, Higashinada-ku, Kobe, Japan.
Transporters have been used in the production of plant metabolites in microorganisms. This study introduced a tobacco multidrug and toxic compound extrusion transporter, NtJAT1, into alkaloid-producing Escherichia coli cells. NtJAT1 expression enhanced alkaloid production secretion into the medium by 14 folds.
View Article and Find Full Text PDFThe gene NtMYC2a acts as a 'master switch' in the regulation of JA-induced nicotine accumulation in tobacco.
Plant Biol (Stuttg)
March 2021
Tobacco Breeding Center, Yunnan Academy of Tobacco Agricultural Sciences, Kunming, China.
The biosynthesis and transport of nicotine has been shown to be coordinately upregulated by jasmonate (JA). MYC2, a member of basic helix-loop-helix (bHLH) transcription factor family, is well-documented as the core player in the JA signalling pathway to regulate diverse plant development processes. Four MYC2 genes were found in the tobacco genome, NtMYC2a/2b and 1a/1b.
View Article and Find Full Text PDFInvolvement of the leaf-specific multidrug and toxic compound extrusion (MATE) transporter Nt-JAT2 in vacuolar sequestration of nicotine in Nicotiana tabacum.
PLoS One
June 2015
Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto University, Kyoto, Japan.
Alkaloids play a key role in higher plant defense against pathogens and herbivores. Following its biosynthesis in root tissues, nicotine, the major alkaloid of Nicotiana species, is translocated via xylem transport toward the accumulation sites, leaf vacuoles. Our transcriptome analysis of methyl jasmonate-treated tobacco BY-2 cells identified several multidrug and toxic compound extrusion (MATE) transporter genes.
View Article and Find Full Text PDFIdentification of a nicotine transporter in leaf vacuoles of Nicotiana tabacum.
Plant Signal Behav
June 2009
Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto University, Gokasho, Uji, Japan.
Alkaloids are, in some plant species, transported from the source organ after their biosynthesis and moved to sink organs via long distance transport. One representative is nicotine, which is biosynthesized in roots, then translocated to the leaves, and finally accumulated in the leaf vacuoles in species. Although the nicotine translocation was identified more than 10 years ago, no transport protein has been characterized concerning the inter-organ movement of this alkaloid.
View Article and Find Full Text PDFVacuolar transport of nicotine is mediated by a multidrug and toxic compound extrusion (MATE) transporter in Nicotiana tabacum.
Proc Natl Acad Sci U S A
February 2009
Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto University, Gokasho, Uji 611-0011, Japan.
Alkaloids play a key role in plant defense mechanisms against pathogens and herbivores, but the plants themselves need to cope with their toxicity as well. The major alkaloid of the Nicotiana species, nicotine, is translocated via xylem transport from the root tissues where it is biosynthesized to the accumulation sites, the vacuoles of leaves. To unravel the molecular mechanisms behind this membrane transport, we characterized one transporter, the tobacco (Nicotiana tabacum) jasmonate-inducible alkaloid transporter 1 (Nt-JAT1), whose expression was coregulated with that of nicotine biosynthetic genes in methyl jasmonate-treated tobacco cells.
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