AI Article Synopsis
- MABC is a significant pathogen for immunocompromised patients, and identifying its subspecies accurately is crucial for effective antibiotic treatment.
- Researchers developed specific PCR assays for MABC subspecies using comparative genomics, which highlighted distinct genetic clusters among the subspecies.
- The study confirmed that these PCR assays are highly sensitive and specific, allowing for reliable identification of MABC subspecies, thus aiding in better diagnosis and treatment decisions.
Article Abstract
complex (MABC) is an important pathogen of immunocompromised patients. Accurate and rapid determination of MABC at the subspecies level is vital for optimal antibiotic therapy. Here we have used comparative genomics to design MABC subspecies-specific PCR assays. Analysis of single nucleotide polymorphisms and core genome multilocus sequence typing showed clustering of genomes into three distinct clusters representing the MABC subspecies , and . Pangenome analysis of 318 MABC genomes from the three subspecies allowed for the identification of 15 MABC subspecies-specific genes. testing of primer sets against 1,663 publicly available MABC genomes and 66 other closely related genomes showed that all assays had >97% sensitivity and >98% specificity. Subsequent experimental validation of two subspecies-specific genes each showed the PCR assays worked well in individual and multiplex format with no false-positivity with 5 other mycobacteria of clinical importance. In conclusion, we have developed a rapid, accurate, multiplex PCR-assay for discriminating MABC subspecies that could improve their detection, diagnosis and inform correct treatment choice.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8995789 | PMC |
| http://dx.doi.org/10.3389/fcimb.2022.816615 | DOI Listing |
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